Determination of S-phenylmercapturic acid in human urine using an automated sample extraction and fast liquid chromatography-tandem mass spectrometric method
| ISSN: | 0269-3879 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1505606fbebcd70849fce0a5d6adbbef https://pubmed.ncbi.nlm.nih.gov/16779771 |
| Rights: | CLOSED |
| Přírůstkové číslo: | edsair.doi.dedup.....1505606fbebcd70849fce0a5d6adbbef |
| Autor: | Weng Naidong, Heiko Junga, Yinghe Li, Haihong Shi, Xiangyu Jiang, John H. Lauterbach, Austin C. Li |
| Rok vydání: | 2006 |
| Předmět: |
Calibration curve
Electrospray ionization Clinical Biochemistry Analytical chemistry Urine Tandem mass spectrometry Biochemistry Sensitivity and Specificity Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Automation Drug Discovery Humans Benzene Molecular Biology Chromatography High Pressure Liquid Pharmacology Chromatography Tandem Selected reaction monitoring General Medicine Reference Standards Acetylcysteine chemistry S-phenylmercapturic acid Female |
| Zdroj: | Biomedical chromatography : BMC. 20(6-7) |
| ISSN: | 0269-3879 |
| Popis: | S-phenylmercapturic acid is widely accepted as a specific biomarker for the evaluation of benzene exposure. Here, we describe a fast, specific and sensitive high-performance liquid achromatography coupled with tandem mass spectrometry (LC-MS/MS) method that has been developed and validated for the determination of S-phenylmercapturic acid in human urine. Isotope-labeled S-phenylmercapturic acid-d5 was used as internal standard to improve the method ruggedness. The fully automated solid-phase extraction method on a 96-well Oasis MAX (mix-mode anion exchange) plate was employed to clean up the urine samples before analysis. The rapid LC-MS/MS analysis of extracted samples was achieved on a Genesis C18 column with a run time of only 3 min. Negative electrospray ionization with multiple reaction monitoring (ESI-MRM) mode was used to detect S-phenylmercapturic acid (m/z 238 --> 109) and S-phenylmercapturic acid -d5 (m/z 243 --> 114). The method fulfils all the standard requirements of method validation. The calibration curve was linear within the concentration range 0.400-200 ng/mL. The method performed accurately and precisely in validation with |
| Databáze: | OpenAIRE |
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