Identification of aerobically and anaerobically induced genes in Enterococcus faecalis by random arbitrarily primed PCR
| Autor: | Michael S. Gilmore, Brett D. Shepard |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Serine-tRNA Ligase
Applied Microbiology and Biotechnology Polymerase Chain Reaction Enterococcus faecalis Microbiology law.invention law Gene expression Methods Humans Anaerobiosis Gene Polymerase chain reaction Gram-Positive Bacterial Infections Genetics Regulation of gene expression Ecology biology Reverse Transcriptase Polymerase Chain Reaction RNA NADH Dehydrogenase Gene Expression Regulation Bacterial biology.organism_classification Streptococcaceae Catalase Aerobiosis RNA Bacterial ATP-Binding Cassette Transporters Oxidoreductases Bacteria Food Science Biotechnology |
| Zdroj: | Applied and environmental microbiology. 65(4) |
| ISSN: | 0099-2240 |
| Popis: | Enterococci have emerged among the leading causes of nosocomial infection. With the goal of analyzing enterococcal genes differentially expressed in environments related to commensal or environmental colonization and infection sites, we adapted and optimized a method more commonly used in the study of eukaryotic gene expression, random arbitrarily primed PCR (RAP-PCR). The RAP-PCR method was systematically optimized, allowing the technique to be used in a highly reproducible manner with gram-positive bacterial RNA. In the present study, aerobiosis was chosen as a variable for the induction of changes in gene expression by Enterococcus faecalis . Aerobically and anaerobically induced genes were detected and identified to the sequence level, and differential gene expression was confirmed by quantitative, specifically primed RT-PCR. Differentially expressed genes included several sharing identity with those of other organisms related to oxygen metabolism, as well as hypothetical genes lacking identity to known genes. |
| Databáze: | OpenAIRE |
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