Whole Genome In-Silico Analysis of South African G1P[8] Rotavirus Strains Before and After Vaccine Introduction Over A Period of 14 Years
| Autor: | Mathew D. Esona, Carl D. Kirkwood, A. Duncan Steele, Ina Peenze, Khuzwayo C. Jere, Martin M. Nyaga, Milton T. Mogotsi, Mapaseka L. Seheri, Benjamin Kumwenda, Valantine N Ndze, M. Jeffrey Mphahlele, Peter N. Mwangi, Francis E. Dennis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
rotavirus strains lineages Wa-like constellation Period (gene) In silico 030106 microbiology Immunology lcsh:Medicine Biology medicine.disease_cause Genome Group A whole-genome 03 medical and health sciences fluids and secretions Rotavirus Drug Discovery evolution medicine Pharmacology (medical) Gene Pharmacology lcsh:R Vaccine introduction Virology 030104 developmental biology Infectious Diseases |
| Zdroj: | Vaccines, Vol 8, Iss 609, p 609 (2020) VACCINES Vaccines Volume 8 Issue 4 |
| Popis: | Rotavirus G1P[8] strains account for more than half of the group A rotavirus (RVA) infections in children under five years of age, globally. A total of 103 stool samples previously characterized as G1P[8] and collected seven years before and seven years after introducing the Rotarix® vaccine in South Africa were processed for whole-genome sequencing. All the strains analyzed had a Wa-like constellation (G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1). South African pre- and post-vaccine G1 strains were clustered in G1 lineage-I and II while the majority (84.2%) of the P[8] strains were grouped in P[8] lineage-III. Several amino acid sites across ten gene segments with the exception of VP7 were under positive selective pressure. Except for the N147D substitution in the antigenic site of eight post-vaccine G1 strains when compared to both Rotarix® and pre-vaccine strains, most of the amino acid substitutions in the antigenic regions of post-vaccine G1P[8] strains were already present during the pre-vaccine period. Therefore, Rotarix® did not appear to have an impact on the amino acid differences in the antigenic regions of South African post-vaccine G1P[8] strains. However, continued whole-genome surveillance of RVA strains to decipher genetic changes in the post-vaccine period remains imperative. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|