Increased angiogenesis and blood vessel maturation in acellular collagen–heparin scaffolds containing both FGF2 and VEGF

Autor: Suzan T.M. Nillesen, Toin H. van Kuppevelt, Willeke F. Daamen, Paul J. Geutjes, Joost Schalkwijk, Ronnie G. Wismans
Rok vydání: 2007
Předmět:
Vascular Endothelial Growth Factor A
Tissue engineering and reconstructive surgery [UMCN 4.3]
Materials science
Angiogenesis
Biophysics
Neovascularization
Physiologic
Biocompatible Materials
Bioengineering
Biomaterials
Andrology
Type IV collagen
Drug Delivery Systems
Implants
Experimental
Tissue engineering
Translational research [ONCOL 3]
In vivo
Materials Testing
medicine
Animals
Rats
Wistar
Cells
Cultured
Renal disorder [IGMD 9]
Bioprosthesis
Cell-Free System
Tissue Engineering
Heparin
Blood Vessel Prosthesis
Extracellular Matrix
Rats
Pathogenesis and modulation of inflammation [N4i 1]
medicine.anatomical_structure
Hypoxia-inducible factors
Mechanics of Materials
Blood vessel maturation
Ceramics and Composites
Blood Vessels
Fibroblast Growth Factor 2
Collagen
Infection and autoimmunity [NCMLS 1]
Immunity
infection and tissue repair [NCMLS 1]
Biomedical engineering
medicine.drug
Blood vessel
Zdroj: Biomaterials, 28, 6, pp. 1123-31
Biomaterials, 28, 1123-31
ISSN: 0142-9612
Popis: Contains fulltext : 51608schalkwijk.pdf (Publisher’s version ) (Closed access) An important issue in tissue engineering is the vascularisation of the implanted construct, which often takes several weeks. In vivo, the growth factors VEGF and FGF2 show a combined effect on both angiogenesis and maturation of blood vessels. Therefore, we hypothesise that the addition of these growth factors to an acellular construct increases blood vessel formation and maturation. To systematically evaluate the contribution of each scaffold component with respect to tissue response and in particular to blood vessel formation, five porous scaffolds were prepared and characterised, viz.: collagen, collagen with heparin, and collagen with heparin plus one or two growth factors (rrFGF2 and rrVEGF). Scaffolds were subcutaneously implanted in 3 months old Wistar rats. Of all scaffolds tested, the one with a combination of growth factors displayed the highest density of blood vessels (type IV collagen) and most mature blood vessels (smooth muscle actin). In addition, no hypoxic cells were found in this scaffold at day 7 and 21 (hypoxia inducible factor 1-alpha). These results indicate that the addition of both FGF2 and VEGF to an acellular construct enhances an early mature vasculature. This opens prospects for (acellular) tissue-engineered constructs in conditions as ischaemic heart disease or diabetic ulcers.
Databáze: OpenAIRE
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