The Serologic Detection of HL-A Antigens in Human Milk
| Autor: | Sandra S. Shasby, D. Bernard Amos, Jeffrey R. Dawson |
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| Rok vydání: | 2008 |
| Předmět: |
Immunology
Spleen Fraction (chemistry) Histocompatibility Testing Biochemistry Serology Isoantibodies fluids and secretions Antigen Histocompatibility Antigens Methods Genetics medicine Animals Humans Immunology and Allergy Centrifugation Lymphocytes Milk Human Chemistry Immune Sera food and beverages Complement System Proteins General Medicine Cytotoxicity Tests Immunologic Chromium Radioisotopes Histocompatibility Phenotype medicine.anatomical_structure Female Rabbits |
| Zdroj: | Tissue Antigens. 4:76-82 |
| ISSN: | 1399-0039 0001-2815 |
| DOI: | 10.1111/j.1399-0039.1974.tb00228.x |
| Popis: | The whey prepared from human milk has been shown to contain the HL-A antigens of the milk donor. The inhibition of specific and nonspecific anti-HL-A alloantisera by the antigens in whey was assessed by a microcytotoxicity assay employing 51Cr release. After centrifugation at 100,000 g for 1 h, significant serologic activity could be demonstrated by the antigens contained in both the insoluble fraction and the soluble fraction. The serologic activity of the HL-A antigens in whey was specific and dose-dependent. The HL-A antigens were not equally represented within the soluble fraction when compared to their distribution in the insoluble fraction, and this may indicate some antigen destruction upon solubilization. |
| Databáze: | OpenAIRE |
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