Selective Cytotoxic Activity of Se-Methyl-Seleno-L-Cysteine- and Se-Polysaccharide-Containing Extracts from Shiitake Medicinal Mushroom, Lentinus edodes (Agaricomycetes)
| Autor: | Emilia Orzechowska, Iwonna Rahden-Staroń, Marzenna Klimaszewska, Emilia Grosicka-Maciag, Piotr Podsadni, Dagmara Kurpios-Piec, Maciej Dawidowski, Agnieszka Szczepańska, Jadwiga Turło, Sandra Górska |
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| Rok vydání: | 2017 |
| Předmět: |
Cell Survival
Shiitake Mushrooms chemistry.chemical_element Antineoplastic Agents Polysaccharide Applied Microbiology and Biotechnology Cell Line HeLa chemistry.chemical_compound 0404 agricultural biotechnology Polysaccharides Cell Line Tumor Drug Discovery Humans Viability assay Pharmacology chemistry.chemical_classification biology Mycelium Biological activity 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Selenocysteine Methylselenocysteine chemistry Biochemistry Polyphenol Lentinus Selenium HeLa Cells |
| Zdroj: | International journal of medicinal mushrooms. 19(8) |
| ISSN: | 1940-4344 |
| Popis: | Numerous formulations derived from the shiitake medicinal mushroom, Lentinus edodes, demonstrate anticancer activities. We hypothesized that isolates from selenium (Se)-enriched mycelia of L. edodes would possess stronger cancer-preventive properties than current preparations. The aim of this study was to investigate whether the presence of Se-methyl-seleno-L-cysteine in mycelial extracts of L. edodes affects their cytotoxic activity (makes them stronger) or whether they are as effective as Se-containing polysaccharides. Extracts were prepared from Se-containing mycelia under various conditions and assayed for cytotoxic activity in cancer (PC3 and HeLa) and normal (HMEC-1) cell lines. The chemical composition of the extracts was examined; specifically, the amounts of potentially cytotoxic Se compounds (methylselenocysteine, selenomethionine, and Se-containing polysaccharides) were measured. The relationship between extract composition and biological activity was characterized. Mycelial cultures were cultivated in a 10-L bioreactor in medium enriched with sodium selenite. Mycelial extracts were prepared either at 100°C or at 4°C in acidic solution. Total Se content was determined using the atomic absorption spectrometry method, and methylselenocysteine and selenomethionine contents were measured using reverse-phase high-performance liquid chromatography. Protein, carbohydrate, and polyphenolic contents were determined with spectrophotometric methods, and Se-containing polysaccharides were measured with the use of precipitation. Anticancer activity of mycelial extracts was examined using the MTT cell viability assay. Extracts containing Se-methyl-seleno-L-cysteine or Se-polysaccharides prepared at 4°C and 100°C, respectively, display moderate, time-dependent, specific cytotoxic activity in HeLa and PC3 cell lines. The effect in HeLa cells is more pronounced in the extract prepared at 4°C than at 100°C. The effect is almost equal for the PC3 cell line. However, both extracts have no effect or only slightly stimulate normal (HMEC-1) cell viability. The selective cytotoxic activity of L. edodes extracts in cancer (PC3 and HeLa) cells is due to the presence of both Se-methyl-seleno-L-cysteine and selenated polysaccharides, perhaps in combination with other active ingredients. |
| Databáze: | OpenAIRE |
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