Abundance, zoonotic potential and risk factors of intestinal parasitism amongst dog and cat populations: The scenario of Crete, Greece

Autor: Despoina Kostopoulou, Smaragda Sotiraki, Nikolaos Voutzourakis, Panagiota Ligda, Edwin Claerebout, Stijn Casaert, Dimitrios Arvanitis
Jazyk: angličtina
Předmět:
0301 basic medicine
Veterinary medicine
Genotyping Techniques
Cat Diseases
Polymerase Chain Reaction
Feces
Zoonoses
Prevalence
KENNELED DOGS
MOLECULAR CHARACTERIZATION
INFECTIOUS-DISEASES
Dog Diseases
Intestinal Diseases
Parasitic

Dipylidium caninum
SHELTER DOGS
Greece
biology
Giardia
Cryptosporidium
Companion animals
030108 mycology & parasitology
Canis
Infectious Diseases
Fluorescent Antibody Technique
Direct

PUBLIC-HEALTH
Cystoisospora
RURAL-AREAS
Parasitic Diseases
Animal

ASCARID INFECTIONS
Risk Assessment
03 medical and health sciences
Dogs
PET DOGS
parasitic diseases
Disease Transmission
Infectious

Animals
Parasites
Veterinary Sciences
GIARDIA-DUODENALIS
Intestinal parasites
Toxascaris leonina
Research
Felis
Zoonotic
Molecular analyses
Sequence Analysis
DNA

biology.organism_classification
FECAL SAMPLES
Ancylostoma
Cats
Parasitology
Zdroj: Parasites & Vectors
PARASITES & VECTORS
ISSN: 1756-3305
DOI: 10.1186/s13071-017-1989-8
Popis: Background The objectives of this study were to evaluate the prevalence and infection intensity of intestinal parasites in different dog and cat populations in Crete, Greece, estimate the zoonotic risk and identify risk factors. Methods Faecal samples from shelter, household and shepherd dogs and shelter and household cats were analyzed using sedimentation/flotation techniques. Giardia and Cryptosporidium were detected by a quantitative direct immunofluorescence assay (IFA). PCR and sequencing was performed to evaluate the zoonotic potential of Giardia and Cryptosporidium positive samples. Results Totals of 879 dog and 264 cat faecal samples were examined. In dogs, the overall prevalence was 25.2% (CI: 22.4–28.1) for Giardia spp.; 9.2% (CI: 7.3–11.1) for Ancylostoma/Uncinaria spp.; 7.6% (CI: 5.9–9.4) for Toxocara spp.; 5.9% (CI: 4.4–7.5) for Cryptosporidium spp.; 4.6% (CI: 3.2–5.9) for Cystoisospora spp.; 2.7% (CI: 1.7–3.8) for Toxascaris leonina; 1.7% (CI: 0.9–2.6) for Capillaria spp.; 0.8% (CI: 0.2–1.4) for taeniid eggs; 0.2% (CI: 0–0.5) for Dipylidium caninum; and 0.1% (CI: 0–0.3) for Strongyloides stercoralis. In cats, the prevalence was 20.5% (CI: 15.6–25.3) for Giardia spp.; 9.5% (CI: 5.9–13.0) for Cystoisospora spp.; 8.3% (CI: 5.0–11.7) for Toxocara spp.; 7.6% (CI: 4.4–10.8) for Ancylostoma/Uncinaria spp.; 6.8% (CI: 3.8–9.9) for Cryptosporidium spp.; 4.2% (CI: 1.8–6.6) for Capillaria spp.; 0.8% (CI: 0–1.8) for taeniid eggs; and 0.4% (CI: 0–1.1) for Hammondia/Toxoplasma. Concerning the risk factors evaluated, there was a negative association between age and Giardia infection and between age and T. leonina infection intensity for dogs. Sequencing results revealed the presence of mainly animal-specific G. duodenalis assemblages C and D in dogs and assemblages F, C and BIV-like in cats, with only a limited number of (co-)infections with assemblage A. As for Cryptosporidium, the dog-specific C. canis and the pig-specific C. scrofarum were detected in dogs and the cat-specific C. felis was detected in cats. Conclusions High levels of parasitism in both dogs and cats were recorded. Giardia was the most prevalent parasite in all dog and cat populations except for shepherd dogs. Genotyping results suggest a limited zoonotic risk of Giardia and Cryptosporidium infections from dogs and cats in Crete. Taeniid eggs were more prevalent in shepherd dogs suggesting access to carcasses and posing a threat for cystic echinococcosis transmission. Infection rates of Toxocara spp. in both dogs and cats show that companion animals could be a significant source of infection to humans. Electronic supplementary material The online version of this article (doi:10.1186/s13071-017-1989-8) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE