Regulation of mPGES‑1 composition and cell growth via the MAPK signaling pathway in jurkat cells
| Autor: | Jieyu Wang, Hong‑Yun Liu, Song‑Mei Yin, Wen‑Juan Yang, Yu‑Dan Wu, Xiu‑Ju Wang, Jiao‑Ting Chen, Li‑Ping Ma, Yi‑Qing Li, Da‑Nian Nie, Zhi‑Yuan He, Shuang‑Feng Xie, Jie Xiao |
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| Rok vydání: | 2018 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine MAPK/ERK pathway Cancer Research Chemistry Kinase Cell growth p38 mitogen-activated protein kinases Articles General Medicine Cell cycle Jurkat cells Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Immunology and Microbiology (miscellaneous) 030220 oncology & carcinogenesis lipids (amino acids peptides and proteins) Signal transduction Protein kinase A |
| Zdroj: | Experimental and Therapeutic Medicine. |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2018.6538 |
| Popis: | Previous studies have suggested that microsomal prostaglandin E synthase-1 (mPGES-1) is highly expressed and closely associated with mitogen-activated protein kinase (MAPK) signaling pathways in various types of malignant cells. However, their expression patterns and function with respect to T-cell acute lymphoblastic leukemia (T-ALL) remain largely unknown. The present study investigated whether mPGES-1 served a crucial role in T-ALL and aimed to identify interactions between mPGES-1 and the MAPK signaling pathway in T-ALL. The results indicated that mPGES-1 overexpression in T-ALL jurkat cells was significantly decreased by RNA silencing. Decreasing mPGES-1 on a consistent basis may inhibit cell proliferation, induce apoptosis and arrest the cell cycle in T-ALL jurkat cells. Microarray and western blot analyses revealed that c-Jun N-terminal kinase served a role in the mPGES-1/prostaglandin E2/EP4/MAPK positive feedback loops. In addition, P38 and extracellular signal-regulated kinase 1/2 exhibited negative feedback effects on mPGES-1. In conclusion, the results suggested that cross-talk between mPGES-1 and the MAPK signaling pathway was very complex. Therefore, the combined regulation of mPGES-1 and the MAPK signaling pathway may be developed into a new candidate therapy for T-ALL in the future. |
| Databáze: | OpenAIRE |
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