Genome-wide organization, evolutionary diversification of the COMMD family genes of amphioxus (Branchiostoma belcheri) with the possible role in innate immunity
| Autor: | Ping Jin, Fei Ma, Shuangli Peng, Caiyun Lv, Lu Cai, Jiu Zhu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
animal structures Aquatic Science Biology Genome Evolution Molecular 03 medical and health sciences 0302 clinical medicine Phylogenetics biology.animal Environmental Chemistry Gene family Animals Amino Acid Sequence Gene Phylogeny Adaptor Proteins Signal Transducing Lancelets Regulation of gene expression Genetics Gene Expression Profiling Intron Vertebrate General Medicine Immunity Innate Gene expression profiling 030104 developmental biology Gene Expression Regulation 030220 oncology & carcinogenesis Multigene Family Sequence Alignment |
| Zdroj: | Fishshellfish immunology. 77 |
| ISSN: | 1095-9947 |
| Popis: | The COMMD (COpper Metabolism gene MURR1 Domain) gene family with ten members participates in various biological processes, such as the regulation of copper and sodium transport, NF-κB activity and cell cycle progression. However, studies on the COMMD gene family in amphioxus (Branchiostoma belcheri) are yet largely unknown. In this study, we have identified and characterized the ten COMMD family members from amphioxus (designated as AmphiCOMMDs). Firstly, we clone the full length of AmphiCOMMDs, and all AmphiCOMMD proteins contain the conserved COMM domain with two NES (Nuclear Export Signal) motifs. Secondly, the genomic structure analysis demonstrates that genes of the COMMD family have undergone intron loss and gain during the process of divergence from amphioxus to vertebrates. Thirdly, phylogenetic analysis indicates that AmphiCOMMDs are more closely related to vertebrates, implying the AmphiCOMMDs may be the ancestor of the vertebrate COMMDs. Fourthly, AmphiCOMMDs are ubiquitously and differentially expressed in five investigated tissues (muscles, gills, intestine, heaptic cecum and notochord). Finally, our results show that expression levels of AmphiCOMMD genes are fluctuating after LPS stimulation to some different extent. Taken together, our studies have elaborated the evolutionary dynamic and the innate immune role of the COMMD family genes in amphioxus. |
| Databáze: | OpenAIRE |
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