Characterization and in vitro studies of the anticancer effect of oxidized carbon nanotubes functionalized with betulinic acid
| Autor: | Govindarajan Karthivashan, Palanisamy Arulselvan, Mohd Zobir Hussein, Sharida Fakurazi, Julia M. Tan |
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| Rok vydání: | 2014 |
| Předmět: |
Thermogravimetric analysis
Biocompatibility Cell Survival Stereochemistry Molecular Conformation Pharmaceutical Science Antineoplastic Agents Carbon nanotube law.invention Mice Structure-Activity Relationship chemistry.chemical_compound law A549 cell line Cell Line Tumor Betulinic acid Drug Discovery Animals Humans Betulinic Acid Cells Cultured Original Research Cell Proliferation HepG2 cell line Pharmacology Drug Design Development and Therapy Nanocomposite Dose-Response Relationship Drug Nanotubes Carbon multiwalled carbon nanotubes (MWCNTs) 3T3 Cells Hep G2 Cells Hydrogen-Ion Concentration Triterpenes chemistry drug delivery Drug delivery cytotoxicity Drug Screening Assays Antitumor Nanocarriers controlled release Pentacyclic Triterpenes Drug carrier Oxidation-Reduction Nuclear chemistry |
| Zdroj: | Drug Design, Development and Therapy |
| ISSN: | 1177-8881 |
| Popis: | Julia M Tan,1 Govindarajan Karthivashan,2 Palanisamy Arulselvan,2 Sharida Fakurazi,2,3 Mohd Zobir Hussein1 1Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 2Laboratory ofVaccine and Immunotherapeutics, Institute of Bioscience (IBS), Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 3Department ofHuman Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Abstract: Among the array of nanomaterials, carbon nanotubes have shown great potential as drug carriers in the field of nanomedicine, owing to their attractive physicochemical structure, which facilitates functionalization of therapeutic molecules onto their external walls or being encapsulated inside the tubes. The aim of this preliminary study was to formulate betulinic acid (BA), a poorly water-soluble drug, in oxidized multiwalled carbon nanotubes (MWCNT-COOH) for enhanced delivery efficiency into cancer cells with reduced cytotoxicity. The synthesized MWCNT-BA nanocomposite was characterized using ultraviolet-visible, Fourier transform infrared, thermogravimetric analysis, powder X-ray diffraction, and field emission scanning electron microscopy techniques. The loading of BA in MWCNT-COOH nanocarrier was estimated to be about 14.5%–14.8% (w/w), as determined by ultraviolet-visible and thermogravimetric analysis. Fourier transform infrared study shows that the peaks of the resulting MWCNT-BA nanocomposite correlate to the characteristic functional groups of BA and MWCNT-COOH. The powder X-ray diffraction results confirmed that the tubular structures of MWCNT-COOH were not affected by the drug loading mechanism of BA. The release profiles demonstrated that approximately 98% of BA could be released within 22hours by phosphate-buffered saline solution at pH 7.4compared with about 22% within 24hours at pH 4.8. The biocompatibility studies revealed that MWCNT-BA at concentrations |
| Databáze: | OpenAIRE |
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