Characterization and in vitro studies of the anticancer effect of oxidized carbon nanotubes functionalized with betulinic acid

Autor: Govindarajan Karthivashan, Palanisamy Arulselvan, Mohd Zobir Hussein, Sharida Fakurazi, Julia M. Tan
Rok vydání: 2014
Předmět:
Thermogravimetric analysis
Biocompatibility
Cell Survival
Stereochemistry
Molecular Conformation
Pharmaceutical Science
Antineoplastic Agents
Carbon nanotube
law.invention
Mice
Structure-Activity Relationship
chemistry.chemical_compound
law
A549 cell line
Cell Line
Tumor

Betulinic acid
Drug Discovery
Animals
Humans
Betulinic Acid
Cells
Cultured

Original Research
Cell Proliferation
HepG2 cell line
Pharmacology
Drug Design
Development and Therapy

Nanocomposite
Dose-Response Relationship
Drug

Nanotubes
Carbon

multiwalled carbon nanotubes (MWCNTs)
3T3 Cells
Hep G2 Cells
Hydrogen-Ion Concentration
Triterpenes
chemistry
drug delivery
Drug delivery
cytotoxicity
Drug Screening Assays
Antitumor

Nanocarriers
controlled release
Pentacyclic Triterpenes
Drug carrier
Oxidation-Reduction
Nuclear chemistry
Zdroj: Drug Design, Development and Therapy
ISSN: 1177-8881
Popis: Julia M Tan,1 Govindarajan Karthivashan,2 Palanisamy Arulselvan,2 Sharida Fakurazi,2,3 Mohd Zobir Hussein1 1Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 2Laboratory ofVaccine and Immunotherapeutics, Institute of Bioscience (IBS), Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 3Department ofHuman Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Abstract: Among the array of nanomaterials, carbon nanotubes have shown great potential as drug carriers in the field of nanomedicine, owing to their attractive physicochemical structure, which facilitates functionalization of therapeutic molecules onto their external walls or being encapsulated inside the tubes. The aim of this preliminary study was to formulate betulinic acid (BA), a poorly water-soluble drug, in oxidized multiwalled carbon nanotubes (MWCNT-COOH) for enhanced delivery efficiency into cancer cells with reduced cytotoxicity. The synthesized MWCNT-BA nanocomposite was characterized using ultraviolet-visible, Fourier transform infrared, thermogravimetric analysis, powder X-ray diffraction, and field emission scanning electron microscopy techniques. The loading of BA in MWCNT-COOH nanocarrier was estimated to be about 14.5%–14.8% (w/w), as determined by ultraviolet-visible and thermogravimetric analysis. Fourier transform infrared study shows that the peaks of the resulting MWCNT-BA nanocomposite correlate to the characteristic functional groups of BA and MWCNT-COOH. The powder X-ray diffraction results confirmed that the tubular structures of MWCNT-COOH were not affected by the drug loading mechanism of BA. The release profiles demonstrated that approximately 98% of BA could be released within 22hours by phosphate-buffered saline solution at pH 7.4compared with about 22% within 24hours at pH 4.8. The biocompatibility studies revealed that MWCNT-BA at concentrations
Databáze: OpenAIRE