Catabolite Regulation of the pta Gene as Part of Carbon Flow Pathways in Bacillus subtilis
| Autor: | Elena Presecan-Siedel, Antoine Danchin, Robert Longin, Philippe Glaser, Anne Galinier, Isabelle Martin-Verstraete, Josef Deutscher |
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| Přispěvatelé: | Institut de biologie et chimie des protéines [Lyon] (IBCP), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Microbiologie et Génétique Moléculaire (MGM), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS), Galinier, Anne |
| Rok vydání: | 1999 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Mutant DNA Footprinting Catabolite repression DNA footprinting Genetics and Molecular Biology macromolecular substances Bacillus subtilis Biology Microbiology Phosphate Acetyltransferase 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Consensus sequence Amino Acid Sequence Phosphate acetyltransferase Phosphoenolpyruvate Sugar Phosphotransferase System Promoter Regions Genetic [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Acetate kinase Base Sequence Acetate Kinase 030306 microbiology Gene Expression Regulation Bacterial Phosphoproteins biology.organism_classification Carbon carbohydrates (lipids) DNA-Binding Proteins Repressor Proteins LOCALISATION DE GENE [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology chemistry Biochemistry CCPA bacteria |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 1999 Journal of Bacteriology, American Society for Microbiology, 1999, 181 (22), pp.6889-6897 Europe PubMed Central |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.181.22.6889-6897.1999 |
| Popis: | In Bacillus subtilis , the products of the pta and ackA genes, phosphotransacetylase and acetate kinase, play a crucial role in the production of acetate, one of the most abundant by-products of carbon metabolism in this gram-positive bacterium. Although these two enzymes are part of the same pathway, only mutants with inactivated ackA did not grow in the presence of glucose. Inactivation of pta had only a weak inhibitory effect on growth. In contrast to pta and ackA in Escherichia coli , the corresponding B. subtilis genes are not cotranscribed. Expression of the pta gene was increased in the presence of glucose, as has been reported for ackA . The effects of the predicted cis -acting catabolite response element (CRE) located upstream from the promoter and of the trans -acting proteins CcpA, HPr, Crh, and HPr kinase on the catabolite regulation of pta were investigated. As for ackA , glucose activation was abolished in ccpA and hprK mutants and in the ptsH1 crh double mutant. Footprinting experiments demonstrated an interaction between CcpA and the pta CRE sequence, which is almost identical to the proposed CRE consensus sequence. This interaction occurs only in the presence of Ser-46-phosphorylated HPr (HPrSer-P) or Ser-46-phosphorylated Crh (CrhSer-P) and fructose-1,6-bisphosphate (FBP). In addition to CcpA, carbon catabolite activation of the pta gene therefore requires at least two other cofactors, FBP and either HPr or Crh, phosphorylated at Ser-46 by the ATP-dependent Hpr kinase. |
| Databáze: | OpenAIRE |
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