Light and Hydrogen Peroxide Inhibit C. elegans Feeding through Gustatory Receptor Orthologs and Pharyngeal Neurons
| Autor: | Nikhil Bhatla, H. Robert Horvitz |
|---|---|
| Přispěvatelé: | Massachusetts Institute of Technology. Department of Biology, Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences, McGovern Institute for Brain Research at MIT, Bhatla, Nikhil, Horvitz, Howard Robert |
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Taste Reactive oxygen species Antioxidant Neuroscience(all) General Neuroscience medicine.medical_treatment Biology Stimulus (physiology) biology.organism_classification chemistry.chemical_compound chemistry Biochemistry medicine Peroxiredoxin Hydrogen peroxide Receptor Caenorhabditis elegans |
| Zdroj: | PMC |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/j.neuron.2014.12.061 |
| Popis: | While gustatory sensing of the five primary flavors (sweet, salty, sour, bitter, and savory) has been extensively studied, pathways that detect non-canonical taste stimuli remain relatively unexplored. In particular, while reactive oxygen species cause generalized damage to biological systems, no gustatory mechanism to prevent ingestion of such material has been identified in any organism. We observed that light inhibits C. elegans feeding and used light as a tool to uncover molecular and neural mechanisms for gustation. Light can generate hydrogen peroxide, and we discovered that hydrogen peroxide similarly inhibits feeding. The gustatory receptor family members LITE-1 and GUR-3 are required for the inhibition of feeding by light and hydrogen peroxide. The I2 pharyngeal neurons increase calcium in response to light and hydrogen peroxide, and these responses require GUR-3 and a conserved antioxidant enzyme peroxiredoxin PRDX-2. Our results demonstrate a gustatory mechanism that mediates the detection and blocks ingestion of a noncanonical taste stimulus, hydrogen peroxide. National Science Foundation (U.S.). Graduate Research Fellowship Program National Institutes of Health (U.S.) (Grant GM24663) |
| Databáze: | OpenAIRE |
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