Disruption of miRNA-mRNA Networks Defines Novel Molecular Signatures for Penile Carcinogenesis
| Autor: | Mauricio Cordeiro, Katia R. M. Leite, Luisa L. Villa, Alexis Murillo Carrasco, Roger Chammas, Giuliano Guglielmetti, Jose Pontes, Laura Sichero, Leonardo Cardilli, Rafael F. Coelho, Miyuki Uno, Claudio Bovolenta Murta, Tatiane Katsue Furuya, William C. Nahas |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Messenger RNA MMP1 Microarray analysis techniques In silico Neoplasms. Tumors. Oncology. Including cancer and carcinogens Computational biology Biology medicine.disease_cause medicine.disease penile cancer Article Oncology microRNA gene expression levels medicine Penile cancer Carcinogenesis Gene carcinogenesis RC254-282 miRNA integrative analysis |
| Zdroj: | Cancers Volume 13 Issue 19 Cancers, Vol 13, Iss 4745, p 4745 (2021) |
| ISSN: | 2072-6694 |
| DOI: | 10.3390/cancers13194745 |
| Popis: | Simple Summary As there are still no biomarkers reported in clinical practice in penile cancer (PeC), we aimed to investigate and validate molecular signatures based on miRNA and mRNA profiles to identify molecular drivers and pathways involved in PeC tumorigenesis. We found eight DEmiRs and 37 DEGs comparing tumoral tissues (TT) paired with non-neoplastic tissues (NNT) of PeC patients. Four downregulated DEmiRs (miR-30a-5p, miR-432-5p, miR-487b-3p, and miR-145-5p) and six upregulated DEGs (IL1A, MCM2, MMP1, MMP12, SFN and VEGFA) were identified as potential biomarkers in PeC by their capacity of discriminating TT and NNT with accuracy. Furthermore, we performed an analysis of miRNA-mRNA interaction and found disruption in the dynamics of the regulation of eight pairs during tumor development that have never been described in PeC. Taken together, our findings contribute to a better understanding of the regulatory roles of miRNAs and altered transcripts levels in penile carcinogenesis. Abstract Penile cancer (PeC) carcinogenesis is not fully understood, and no biomarkers are reported in clinical practice. We aimed to investigate molecular signatures based on miRNA and mRNA and perform an integrative analysis to identify molecular drivers and pathways for PeC development. Affymetrix miRNA microarray was used to identify differentially expressed miRNAs (DEmiRs) comparing 11 tumoral tissues (TT) paired with non-neoplastic tissues (NNT) with further validation in an independent cohort (n = 13). We also investigated the mRNA expression of 83 genes in the total sample. Experimentally validated targets of DEmiRs, miRNA-mRNA networks, and enriched pathways were evaluated in silico. Eight out of 69 DEmiRs identified by microarray analysis were validated by qRT-PCR (miR-145-5p, miR-432-5p, miR-487b-3p, miR-30a-5p, miR-200a-5p, miR-224-5p, miR-31-3p and miR-31-5p). Furthermore, 37 differentially expressed genes (DEGs) were identified when comparing TT and NNT. We identified four downregulated DEmiRs (miR-30a-5p, miR-432-5p, miR-487b-3p, and miR-145-5p) and six upregulated DEGs (IL1A, MCM2, MMP1, MMP12, SFN and VEGFA) as potential biomarkers in PeC by their capacity of discriminating TT and NNT with accuracy. The integration analysis showed eight dysregulated miRNA-mRNA pairs in penile carcinogenesis. Taken together, our findings contribute to a better understanding of the regulatory roles of miRNAs and altered transcripts levels in penile carcinogenesis. |
| Databáze: | OpenAIRE |
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