Detection of t(14;18) carrying cells in bone marrow and peripheral blood from patients affected by non-lymphoid diseases
| Autor: | J J Chale, Georges Delsol, T al Saati, D Adoue, J.L. Albarede, Sandrine Galoin, O Rauzy |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Lymphoma Clone (cell biology) Chromosomal translocation Immunoglobulin E Polymerase Chain Reaction Translocation Genetic Pathology and Forensic Medicine Age Distribution Chromosome 18 Bone Marrow medicine Humans Lymphocytes Aged Aged 80 and over Chromosomes Human Pair 14 biology Middle Aged medicine.disease Genes bcl-2 medicine.anatomical_structure Peripheral blood lymphocyte Immunology biology.protein Female Bone marrow Antibody Chromosomes Human Pair 18 Immunoglobulin Heavy Chains Research Article |
| Zdroj: | Molecular pathology : MP. 51(6) |
| ISSN: | 1366-8714 |
| Popis: | AIMS/BACKGROUND: To assess the presence of bcl-2/JH rearrangements in bone marrow and peripheral blood lymphocytes from patients affected by diseases other than malignant lymphomas. The t(14;18) (q32;q21) translocation, which juxtaposes the bcl-2 oncogene on chromosome 18 and the JH segment of the immunoglobulin heavy chain (IgH) genes on chromosome 14, is found frequently in follicular lymphomas. METHODS: A sensitive semi-nested polymerase chain reaction (PCR) was used to detect t(14;18) translocation in bone marrow aspirates and peripheral blood lymphocytes from 48 patients. In 137 additional individuals peripheral blood lymphocytes only were tested. RESULTS: Cells carrying bcl-2/JH rearrangements were detected in about a quarter of the bone marrow samples and half of the peripheral blood lymphocyte samples. In seven patients, t(14;18) positive cells were found in both the bone marrow and peripheral blood lymphocyte samples. The size of the PCR products and bcl-2/JH DNA sequence analysis showed that the same t(14;18) carrying clone was present in the bone marrow and the corresponding peripheral blood lymphocyte samples in three of these seven patients. Some patients had more than one bcl-2/JH rearrangement. There was no significant correlation between age and the translocation incidence. Cells carrying the t(14;18) translocation were present in peripheral blood lymphocyte samples with a similar incidence--between 47% and 52% in all age groups from 20 to 79 years. Patients older than 80 years had a lower (37%) but not significantly different incidence. CONCLUSIONS: These findings suggest that patients affected by non-lymphoid diseases may have several t(14;18) carrying cells and some of them undergo a clonal expansion. Whether individuals with t(14;18) positive cells are at a higher risk of lymphoid malignancies remains unanswered and further epidemiological studies are required. |
| Databáze: | OpenAIRE |
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