Gene Network Analysis of Interstitial Macrophages After Treatment with Induced Pluripotent Stem Cells Secretome (iPSC-cm) in the Bleomycin Injured Rat Lung
| Autor: | Torsten Goldmann, Mathias Gugger, Thomas Geiser, Anis Feki, Luca Tamò, Cedric Simillion, Benedikt Jäger, Antje Prasse, Youssef Hibaoui, Amiq Gazdhar |
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| Přispěvatelé: | Publica |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine induced pluripotent stem cell Cancer Research Stem cell secretome Induced Pluripotent Stem Cells 610 Medicine & health macrophage Biology Lung injury Bleomycin Article 03 medical and health sciences Idiopathic pulmonary fibrosis chemistry.chemical_compound Fibrosis medicine Animals Humans Gene Regulatory Networks Induced pluripotent stem cell Lung Cells Cultured Macrophages Stem Cells Wnt signaling pathway Cell Biology respiratory system Flow Cytometry medicine.disease Rats respiratory tract diseases 030104 developmental biology medicine.anatomical_structure chemistry Cancer research Lung fibrosis Stem cell |
| Zdroj: | Tamò, Luca Giuseppe Athos; Simillion, Cedric; Hibaoui, Youssef; Feki, Anis; Gugger, Mathias; Prasse, Antje; Jäger, Benedikt; Goldmann, Torsten; Geiser, Thomas; Gazdhar, Amiq (2018). Gene Network Analysis of Interstitial Macrophages After Treatment with Induced Pluripotent Stem Cells Secretome (iPSC-cm) in the Bleomycin Injured Rat Lung. Stem cell reviews and reports, 14(3), pp. 412-424. Springer 10.1007/s12015-017-9790-9 Stem Cell Reviews |
| ISSN: | 1558-6804 1550-8943 |
| DOI: | 10.1007/s12015-017-9790-9 |
| Popis: | Idiopathic pulmonary fibrosis (IPF) is a complex disease involving various cell types. Macrophages are essential in maintenance of physiological homeostasis, wound repair and fibrosis in the lung. Macrophages play a crucial role in repair and remodeling by altering their phenotype and secretory pattern in response to injury. The secretome of induced pluripotent stem cells (iPSC-cm) attenuates injury and fibrosis in bleomycin injured rat lungs. In the current study, we evaluate the effect of iPSC-cm on gene expression and phenotype of interstitial macrophage in bleomycin injured rat lungs in vivo. iPSC-cm was intratracheally instilled 7 days after bleomycin induced lung injury and assessed 7 days later and single cell isolation was performed. Macrophages were FACS sorted and microarray analysis was performed. We characterized changes in the rat lung interstitial macrophages using transcriptional profiling. iPSC-cm reduced the total collagen content of the lung and reduced different macrophage populations. Gene set enrichment analysis revealed involvement of three essential pathways (a) immune modulation, (b) branching morphogenesis and (c) canonical Wnt signaling. This study demonstrates that iPSC-cm reduces fibrosis in bleomycin injured rat lung by partially altering the macrophages and regulating their gene expression. Electronic supplementary material The online version of this article (10.1007/s12015-017-9790-9) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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