Separation of two sub-groups with different DNA content after treatment of T-47D breast cancer cells with low dose-rate irradiation and intermittent hypoxia
| Autor: | Nina Jeppesen Edin, Stine Gyland Mikalsen, Erik O. Pettersen, Joe Alexander Sandvik |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival Population Breast Neoplasms Radiation Dosage Radiation Tolerance Metastasis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor Medicine Humans Radiology Nuclear Medicine and imaging Radiosensitivity Irradiation education Cloning education.field_of_study Radiological and Ultrasound Technology business.industry Intermittent hypoxia General Medicine DNA Hypoxia (medical) medicine.disease Molecular biology Cell Hypoxia 030104 developmental biology chemistry 030220 oncology & carcinogenesis Immunology Female medicine.symptom business |
| Zdroj: | Acta radiologica (Stockholm, Sweden : 1987). 59(1) |
| ISSN: | 1600-0455 |
| Popis: | Background Previous studies have shown that combined treatment with internal ultra-low dose-rate irradiation selectively inactivated hypoxic T–47D breast cancer cells after three to five weeks of treatment. However, 2–3% of the hypoxic cells were found to survive and restart proliferation upon re-oxygenation. Purpose To investigate the metastatic potential and characteristics of radiosensitivity of these surviving cells, named T – 47 DS. Material and Methods The T – 47 DS cells were grown in ambient air without irradiation. A cloning experiment identified two sub-groups with different DNA content ([Formula: see text] and [Formula: see text]). Furthermore, radiosensitivity and presence of hyper-radiosensitivity (HRS) was measured by Co-60 challenge irradiation and relative migration was determined by scratch assays. Results The two subpopulations of T – 47 DS had different DNA content; one had abnormally high DNA content ([Formula: see text]) and one had DNA content similar to wild-type T–47D cells ([Formula: see text]). HRS was surprisingly present in cells of the cloned population [Formula: see text], but was absent in cells of both [Formula: see text] and T – 47 DS. The radio response of T – 47 DS, [Formula: see text] at higher radiation doses were similar to that of T-47D cells, and neither subpopulation showed increased migration compared with wild-type T–47D. Conclusion No increase in the risk of metastasis was found and only slight changes in radiosensitivity in response to conventional clinical doses was observed. Thus, the data suggest that if ultra-low dose-rate irradiation is used for targeting the hypoxic tumor fraction, conventional high dose-rate irradiation can be used to eradicate eventual surviving cells as well as cells in the well oxygenated areas of the tumor. |
| Databáze: | OpenAIRE |
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