Ability of matrix metalloproteinase-8 biosensor, IFMA, and ELISA immunoassays to differentiate between periodontal health, gingivitis, and periodontitis
| Autor: | Kehinde Adesola Umeizudike, Hanna Lähteenmäki, Ismo T Räisänen, John J Taylor, Philip M Preshaw, Susan M Bissett, Taina Tervahartiala, Solomon Nwhator, Pirjo Pärnänen, Timo Sorsa |
|---|---|
| Přispěvatelé: | Department of Oral and Maxillofacial Diseases, University of Helsinki, HUS Head and Neck Center, Clinicum |
| Rok vydání: | 2022 |
| Předmět: |
TISSUE DESTRUCTION
active/total matrix metalloproteinase-8 saliva CREVICULAR FLUID periodontal disease INTERSTITIAL COLLAGENASES Fluorescent Antibody Technique MOUTHRINSE active Enzyme-Linked Immunosorbent Assay Biosensing Techniques biosensor DIAGNOSIS CLASSIFICATION Antibodies Humans NEUTROPHIL COLLAGENASE ACTIVITY Periodontitis Saliva Immunoassay GLOBAL BURDEN Gingivitis 313 Dentistry Matrix Metalloproteinase 8 total matrix metalloproteinase-8 ADULT PERIODONTITIS Periodontics MMP-8 Biomarkers |
| Zdroj: | Journal of periodontal research. 57(3) |
| ISSN: | 1600-0765 |
| Popis: | Publisher Copyright: © 2022 The Authors. Journal of Periodontal Research published by John Wiley & Sons Ltd. Objective: The aim of this study was to determine the diagnostic utility of an MMP-8 biosensor assay in differentiating periodontal health from gingivitis and periodontitis and compare it with an established time-resolved immunofluorescence assay (IFMA) and enzyme-linked immunosorbent assay (ELISA). Background: Currently available antibody-based assays display a wide variability in their ability to accurately measure matrix metalloproteinase-8 (MMP-8) levels in saliva. Methods: Salivary MMP-8 levels were analyzed in 189 systemically healthy participants using an antibody-based biosensor prototype that operates using a surface acoustic wave technology and compared with IFMA and ELISA antibody assays. Participants were categorized into 3 groups: periodontal health (59), gingivitis (63), and periodontitis (67). A sub-population of participants (n = 20) with periodontitis received periodontal treatment and were monitored for 6 months. Results: All the assays demonstrated significantly higher salivary MMP-8 concentrations in participants with periodontitis versus gingivitis, periodontitis versus health, and gingivitis versus health (all p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text