Characterisation of the manganese superoxide dismutase of Enterococcus faecium
| Autor: | Aurélie Budin-Verneuil, Eliette Riboulet-Bisson, Axel Hartke, Valentin Wasselin, Cindy Staerck, Abdellah Benachour, Loïc Léger, Isabelle Rincé |
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| Rok vydání: | 2021 |
| Předmět: |
Enterococcus mundtii
Enterococcus faecium Moths Microbiology Antioxidants Superoxide dismutase chemistry.chemical_compound Menadione Bacterial Proteins Enterococcus hirae Superoxides Benzene Derivatives Animals Molecular Biology Phylogeny biology Virulence Superoxide Superoxide Dismutase food and beverages General Medicine Hydrogen Peroxide biochemical phenomena metabolism and nutrition biology.organism_classification Enterococcus durans Aerobiosis Oxidative Stress chemistry Cumene hydroperoxide Enzyme Induction biology.protein bacteria Genome Bacterial |
| Zdroj: | Research in microbiology. 172(6) |
| ISSN: | 1769-7123 |
| Popis: | The manganese superoxide dismutase (SodA) of E. faecium strain AUS0004 has been characterised. It is most closely related to Enterococcus hirae , Enterococcus durans , Enterococcus villorium, and Enterococcus mundtii with 100%, 91,55%, 90,85%, and 90,58% homology, respectively, but more distant from SodA of E. faecalis (81.68%). A sodA deletion mutant has been constructed. Compared to the parental strain, the ΔsodA mutant was affected in aerobic growth and more sensitive to hydrogen peroxide (H2O2), cumene hydroperoxide (CuOOH), and the superoxide anion (O2•-) generator menadione. The E. faecium strain AUS0004 is part of those bacteria accumulating H2O2 to high concentrations (around 5 mM) starting from late exponential growth phase. Accumulation of the peroxide was around 25% less in the mutant suggesting that this part of H2O2 is due to the dismutation of O2•- by SodA. The sodA gene of E. faecium AUS0004 was induced by oxygen, peroxides and menadione but the corresponding regulator remains hitherto unknown. Finally, we showed that SodA activity is important for virulence in the Galleria mellonella model. |
| Databáze: | OpenAIRE |
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