Evaluation of Semiautomated Multiplex PCR Assay for Determination of Streptococcus pneumoniae Serotypes and Serogroups
| Autor: | Siobhan Martin, Robert George, Lucinda M. C. Hall, Elliot R. Lawrence, David B. Griffiths |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Quality Control
Microbiology (medical) Serotype Single sample Locus (genetics) medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention Fragment size Automation law Streptococcus pneumoniae Multiplex polymerase chain reaction medicine Humans Serotyping Polymerase chain reaction DNA Primers biology Electrophoresis Capillary Bacteriology Streptococcaceae biology.organism_classification Virology Reagent Kits Diagnostic |
| Zdroj: | Journal of Clinical Microbiology. 41:601-607 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.41.2.601-607.2003 |
| Popis: | A semiautomated method for the determination of five serotypes and three serogroups in Streptococcus pneumoniae was developed. Primers specific for serotypes 1, 3, 14, 19F, and 23F and serogroups 6, 19, and 23 were combined in three multiplex PCRs. Products were separated by capillary electrophoresis with a 7-min run time, and a serotype or serogroup was assigned on the basis of fragment size. The method was used to test 93 clinical isolates, and all isolates of the serotypes concerned were correctly detected. The strategy would allow the detection of multiple serotypes in a single sample. Detection of additional serotypes could be included as capsule locus sequences become available. |
| Databáze: | OpenAIRE |
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