The application of protein markers in conversion of maize inbred lines to the cytoplasmic male sterility basis
| Autor: | Jovan Pavlov, Jelena Vančetović, Zoran Camdzija, Milomir Filipović, Milan Stevanovic, Snezana Mladenovic-Drinic, Ksenija Markovic |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics lcsh:QH426-470 grain yield Cytoplasmic male sterility Plant Science Biology male sterility 01 natural sciences Protein markers isoelectric focusing (IEF) lcsh:Genetics 03 medical and health sciences 030104 developmental biology Inbred strain banding patterns health services administration Grain yield health care economics and organizations 010606 plant biology & botany |
| Zdroj: | Genetika Genetika, Vol 48, Iss 2, Pp 691-698 (2016) |
| Popis: | A total of seven maize inbred lines of different origin and maturity group were used in the trial set up according to the split-plot randomized complete block design in five environments. Each inbred was observed in five variants: original inbred (N); cytoplasmic male sterile C-type (CMS-C); restorer for CMS-C (RfC); cytoplasmic male sterile S-type (CMS-S) and restorer for CMS-S (RfS). The objective was to compare grain yield of original inbreds and their CMS and Rf variants and to apply Isoelectric focusing (IEF) to determine whether the conversion of original inbreds to their CMS and Rf counterparts have been done completely. Protein markers have shown that conversion of almost all inbreds was done good and completely. Only original inbreds ZPL2 and ZPL5 did not concur on banding patterns with their RfC variants. The type of cytoplasm had a very significant impact on grain yield. Namely, CMS-C counterparts significantly out yielded their CMS-S versions, while the inbreds with C and S cytoplasm over yielded inbreds with N cytoplasm, as well as their RfC and RfS versions. |
| Databáze: | OpenAIRE |
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