Headspace solid-phase microextraction (HS-SPME) combined with GC–MS as a process analytical technology (PAT) tool for monitoring the cultivation of C. tetani
| Autor: | Ali Es-haghi, Masoud Ghader, Nader Shokoufi, Kazem Kargosha |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Central composite design
Clostridium tetani Process analytical technology Clinical Biochemistry Vaccine Production Solid-phase microextraction medicine.disease_cause 01 natural sciences Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry Bioreactors medicine Bioreactor Solid Phase Microextraction Volatile Organic Compounds Chromatography 010405 organic chemistry Chemistry 010401 analytical chemistry Cell Biology General Medicine 0104 chemical sciences Sampling time Gas chromatography–mass spectrometry |
| Zdroj: | Journal of Chromatography B. 1083:222-232 |
| ISSN: | 1570-0232 |
| Popis: | Vaccine production is a biological process in which variation in time and output is inevitable. Thus, the application of Process Analytical Technologies (PAT) will be important in this regard. Headspace solid - phase microextraction (HS-SPME) coupled with GC–MS can be used as a PAT for process monitoring. This method is suitable to chemical profiling of volatile organic compounds (VOCs) emitted from microorganisms. Tetanus is a lethal disease caused by Clostridium tetani (C. tetani) bacterium and vaccination is an ultimate way to prevent this disease. In this paper, SPME fiber was used for the investigation of VOCs emerging from C. tetani during cultivation. Different types of VOCs such as sulfur-containing compounds were identified and some of them were selected as biomarkers for bioreactor monitoring during vaccine production. In the second step, the portable dynamic air sampling (PDAS) device was used as an interface for sampling VOCs by SPME fibers. The sampling procedure was optimized by face-centered central composite design (FC-CCD). The optimized sampling time and inlet gas flow rates were 10 min and 2 m L s−1, respectively. PDAS was mounted in exhausted gas line of bioreactor and 42 samples of VOCs were prepared by SPME fibers in 7 days during incubation. Simultaneously, pH and optical density (OD) were evaluated to cultivation process which showed good correlations with the identified VOCs (>80%). This method could be used for VOCs sampling from off-gas of a bioreactor to monitoring of the cultivation process. |
| Databáze: | OpenAIRE |
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