Pfcyp51 exclusively determines reduced sensitivity to 14α-demethylase inhibitor fungicides in the banana black Sigatoka pathogen Pseudocercospora fijiensis
Autor: | Pablo Chong, Aikaterini Eleni Vichou, Rafael Arango Isaza, Harold J. G. Meijer, Gert H. J. Kema, Henk J. Schouten |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Leaves
Black sigatoka Heredity Genetic Linkage Physical Mapping Plant Science Bananas Gene mutation Genetic analysis Sterol 14-Demethylase Promoter Regions Genetic Fungicides Genetics education.field_of_study Multidisciplinary Plant Anatomy Fungal genetics Eukaryota Agriculture Plants 14-alpha Demethylase Inhibitors Medicine Agrochemicals PBR Biodiversity and genetic variation Research Article Science Population Mycology Biology Research and Analysis Methods Fruits Fungal Proteins Biointeractions and Plant Health PBR Biodiversiteit en Genetische Variatie Gene mapping Ascomycota Drug Resistance Fungal Life Science Fungal Genetics education Molecular Biology Techniques Linkage Mapping Gene Molecular Biology Plant Diseases Gene Mapping Organisms Biology and Life Sciences Musa Laboratorium voor Phytopathologie Fungicides Industrial Plant Leaves Plant Breeding Genetic marker Laboratory of Phytopathology Mutation EPS human activities |
Zdroj: | PLoS ONE, Vol 14, Iss 10, p e0223858 (2019) PLoS ONE PLoS ONE 14 (2019) 10 PLoS ONE, 14(10) |
ISSN: | 1932-6203 |
Popis: | The haploid fungus Pseudocercospora fijiensis causes black Sigatoka in banana and is chiefly controlled by extensive fungicide applications, threatening occupational health and the environment. The 14α-Demethylase Inhibitors (DMIs) are important disease control fungicides, but they lose sensitivity in a rather gradual fashion, suggesting an underlying polygenic genetic mechanism. In spite of this, evidence found thus far suggests that P. fijiensis cyp51 gene mutations are the main responsible factor for sensitivity loss in the field. To better understand the mechanisms involved in DMI resistance, in this study we constructed a genetic map using DArTseq markers on two F1 populations generated by crossing two different DMI resistant strains with a sensitive strain. Analysis of the inheritance of DMI resistance in the F1 populations revealed two major and discrete DMI-sensitivity groups. This is an indicative of a single major responsible gene. Using the DMI-sensitivity scorings of both F1 populations and the generation of genetic linkage maps, the sensitivity causal factor was located in a single genetic region. Full agreement was found for genetic markers in either population, underlining the robustness of the approach. The two maps indicated a similar genetic region where the Pfcyp51 gene is found. Sequence analyses of the Pfcyp51 gene of the F1 populations also revealed a matching bimodal distribution with the DMI resistant. Amino acid substitutions in P. fijiensis CYP51 enzyme of the resistant progeny were previously correlated with the loss of DMI sensitivity. In addition, the resistant progeny inherited a Pfcyp51 gene promoter insertion, composed of a repeat element with a palindromic core, also previously correlated with increased gene expression. This genetic approach confirms that Pfcyp51 is the single explanatory gene for reduced sensitivity to DMI fungicides in the analysed P. fijiensis strains. Our study is the first genetic analysis to map the underlying genetic factors for reduced DMI efficacy. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |