Culture degeneration in conidia of Beauveria bassiana and virulence determinants by proteomics
| Autor: | Jiraporn Jirakkakul, Suthathip Kittisenachai, Laihong So, Juntira Punya, Janthima Jaresitthikunchai, Chettida Srisuksam, Alongkorn Amnuaykanjanasin, Jittisak Senachak, Pratchya Swangmaneecharern, Morakot Tanticharoen, Songsak Wattanachaisaereekul, Anuwat Tachaleat, Peerada Prommeenate, Supapon Cheevadhanarak, Sittiruk Roytrakul |
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| Rok vydání: | 2018 |
| Předmět: |
DNA Replication
Proteomics 0301 basic medicine 030106 microbiology Virulence Beauveria bassiana Bassiana Spodoptera Biology Microbiology Conidium 03 medical and health sciences Amino acid homeostasis Autophagy Genetics Animals Beauveria Ecology Evolution Behavior and Systematics TOR Serine-Threonine Kinases fungi Spores Fungal biology.organism_classification Circadian Rhythm Oxidative Stress Phenotype 030104 developmental biology Infectious Diseases Potato dextrose agar Subculture (biology) Signal Transduction |
| Zdroj: | Fungal Biology. 122:156-171 |
| ISSN: | 1878-6146 |
| Popis: | The quality of Beauveria bassiana conidia directly affects the virulence against insects. In this study, continuous subculturing of B. bassiana on both rice grains and potato dextrose agar (PDA) resulted in 55 and 49 % conidial yield reduction after 12 passages and 68 and 60 % virulence reduction after 20 and 12 passages at four d post-inoculation, respectively. The passage through Tenebrio molitor and Spodoptera exigua restored the virulence of rice and PDA subcultures, respectively. To explore the molecular mechanisms underlying the conidial quality and the decline of virulence after multiple subculturing, we investigated the conidial proteomic changes. Successive subculturing markedly increased the protein levels in oxidative stress response, autophagy, amino acid homeostasis, and apoptosis, but decreased the protein levels in DNA repair, ribosome biogenesis, energy metabolism, and virulence. The nitro blue tetrazolium assay verified that the late subculture's colony and conidia had a higher oxidative stress level than the early subculture. A 2A-type protein phosphatase and a Pleckstrin homology domain protein Slm1, effector proteins of the target of rapamycin (TOR) complex 1 and 2, respectively, were dramatically increased in the late subculture. These results suggest that TOR signalling might be associated with ageing in B. bassiana late subculture, in turn affecting its physiological characteristics and virulence. |
| Databáze: | OpenAIRE |
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