Different interaction between HIV-1 Vif and its cellular target proteins APOBEC3G/APOBEC3F
| Autor: | Masako Nomaguchi, Tomoki Yamashita, Akio Adachi, Tsuneo Uchiyama, Tamiko Nagao, Ariko Miyake |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Infectivity
viruses Mutant Human immunodeficiency virus (HIV) virus diseases APOBEC-3G Deaminase General Medicine biochemical phenomena metabolism and nutrition Biology medicine.disease_cause Virology Vif General Biochemistry Genetics and Molecular Biology Cell Line Cytosine Deaminase Equilibrium density Cytidine Deaminase vif Gene Products Human Immunodeficiency Virus HIV-1 medicine Humans Centrifugation APOBEC3G APOBEC3F |
| Zdroj: | The Journal of Medical Investigation. 57:89-94 |
| ISSN: | 1349-6867 1343-1420 |
| DOI: | 10.2152/jmi.57.89 |
| Popis: | We examined a series of site-directed point mutants of human immunodeficiency virus type 1 (HIV-1) Vif for their interaction with cellular anti-viral factors APOBEC3G/APOBEC3F. Mutant viruses that display growth-defect in H9 cells did not counteract effectively APOBEC3G and/or APOBEC3F without exception, as monitored by single-cycle infectivity assays. While growth-defective mutants of Vif C-terminal region were unable to suppress APOBEC3G/APOBEC3F, some N-terminal region mutants did neutralize one of APOBEC3G/APOBEC3F. These data have suggested that members of APOBEC3 family other than APOBEC3G/APOBEC3F are not important for anti-HIV-1 activity. Furthermore, APOPEC3G/APOBEC3F were found to differently associate with Vif in virions as analyzed by equilibrium density centrifugation. Taken together, these results indicated that interaction of HIV-1 Vif and APOBEC3G is distinct from that between Vif and APOBEC3F. |
| Databáze: | OpenAIRE |
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