The Arabidopsis Iron-Sulfur (Fe-S) Cluster Gene
| Autor: | David V. Huhman, Sunhee Oh, Bonnie S. Watson, Clarissa Boschiero, Kirankumar S. Mysore, Jose Pedro Fonseca |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Iron-Sulfur Proteins Mutant Arabidopsis 01 natural sciences Plant defense against herbivory Pseudomonas syringae Plant Immunity Biology (General) Spectroscopy Disease Resistance biology food and beverages General Medicine ferredoxin Computer Science Applications Cell biology Mitochondria Chemistry Multigene Family MFDX1 Ferredoxins abiotic stress QH301-705.5 Iron Glucosinolates NFS1 Plant disease resistance Article Catalysis Inorganic Chemistry 03 medical and health sciences biotic stress Stress Physiological Physical and Theoretical Chemistry Molecular Biology Gene QD1-999 Plant Diseases Abiotic stress Arabidopsis Proteins Organic Chemistry fungi Biotic stress biology.organism_classification 030104 developmental biology Sulfur 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 7147, p 7147 (2021) International Journal of Molecular Sciences Volume 22 Issue 13 |
| ISSN: | 1422-0067 |
| Popis: | Until recently, genes from the iron-sulfur (Fe-S) cluster pathway were not known to have a role in plant disease resistance. The Nitrogen Fixation S (NIFS)-like 1 (NFS1) and Mitochondrial Ferredoxin-1 (MFDX1) genes are part of a set of 27 Fe-S cluster genes induced after infection with host and nonhost pathogens in Arabidopsis. A role for AtNFS1 in plant immunity was recently demonstrated. In this work, we showed that MFDX1 is also involved in plant defense. More specifically, Arabidopsis mfdx1 mutants were compromised for nonhost resistance against Pseudomonas syringae pv. tabaci, and showed increased susceptibility to the host pathogen P. syringae pv. tomato DC3000. Arabidopsis AtMFDX1 overexpression lines were less susceptible to P. syringae pv. tomato DC3000. Metabolic profiling revealed a reduction of several defense-related primary and secondary metabolites, such as asparagine and glucosinolates in the Arabidopsis mfdx1-1 mutant when compared to Col-0. A reduction of 5-oxoproline and ornithine metabolites that are involved in proline synthesis in mitochondria and affect abiotic stresses was also observed in the mfdx1-1 mutant. In contrast, an accumulation of defense-related metabolites such as glucosinolates was observed in the Arabidopsis NFS1 overexpressor when compared to wild-type Col-0. Additionally, mfdx1-1 plants displayed shorter primary root length and reduced number of lateral roots compared to the Col-0. Taken together, these results provide additional evidence for a new role of Fe-S cluster pathway in plant defense responses. |
| Databáze: | OpenAIRE |
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