Carbendazim exposure during the larval stage suppresses major royal jelly protein expression in nurse bees (Apis mellifera)
| Autor: | Fuchao Gao, Zheguang Lin, Ting Ji, Zhi Wang, Kang Wang, Qing-Sheng Niu, Heng Chen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Environmental Engineering food.ingredient Cell division Health Toxicology and Mutagenesis 0208 environmental biotechnology 02 engineering and technology 010501 environmental sciences Biology 01 natural sciences Colony collapse disorder chemistry.chemical_compound food Nursing Downregulation and upregulation Royal jelly Protein biosynthesis Animals Environmental Chemistry Mitosis 0105 earth and related environmental sciences Carbendazim Fatty Acids Public Health Environmental and Occupational Health General Medicine General Chemistry Bees Pollution 020801 environmental engineering Fungicide chemistry Larva Insect Proteins Benzimidazoles Carbamates |
| Zdroj: | Chemosphere. 266:129011 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2020.129011 |
| Popis: | Studying the sublethal effects of agrochemical pesticides on nontarget honeybees (Apis mellifera) is important for agricultural development. Carbendazim is a widely used broad-spectrum fungicide that inhibits mitotic microtubule formation and cell division. However, the impact of carbendazim on bee health and development has not been fully elucidated. Here, using proteomics approaches, we assessed in vitro the changes in the expression of functional proteins in the head of newly emerged adults following treatment with field concentration of carbendazim during the larval stage. Treatment with carbendazim severely altered 266 protein expression patterns in the heads of adults and 218 of them showed downregulation after carbendazim exposure. Notably, major royal jelly proteins, a crucial multifunctional protein family with irreplaceable function in sustaining the development of colonies, were significantly suppressed in carbendazim-treated bees. This result was verified in both head and hypopharyngeal gland of nurse bees. Moreover, visual and olfactory loss, immune functions, muscular activity, social behavior, neural and brain development, protein synthesis and modification, and metabolism-related proteins were likely inhibited by carbendazim treatment. Together, these results suggest that carbendazim is an environmental risk factor that likely weakens bee colonies, partially due to reduced expression of major royal jelly proteins, which may be potential causes of colony collapse disorder. |
| Databáze: | OpenAIRE |
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