Nanoscale Changes on RBC Membrane Induced by Storage and Ionizing Radiation: A Mini-Review
| Autor: | Aracely Angulo-Molina, Adriana Garibay-Escobar, Mónica Acosta-Elías, Karla Santacruz-Gómez, Miguel A. Méndez-Rojas, Beatriz Castañeda-Medina, Andrea M López-Canizales, Osiris Álvarez-Bajo, Alexel Burgara-Estrella, Erika Silva-Campa, Martín Pedroza-Montero, Diego Soto-Puebla |
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| Rok vydání: | 2021 |
| Předmět: |
atomic force microscopy
Physiology Chemistry Mini Review Bilayer Vesicle nanoalterations blood storage Confocal scanning microscopy confocal microscopy RBC membrane law.invention Membrane Confocal microscopy law Physiology (medical) Biophysics QP1-981 ionizing radiation Lipid bilayer Cytoskeleton Raman Cell aging scanning electron microscopy |
| Zdroj: | Frontiers in Physiology, Vol 12 (2021) Frontiers in Physiology |
| ISSN: | 1664-042X |
| DOI: | 10.3389/fphys.2021.669455 |
| Popis: | The storage lesions and the irradiation of blood cellular components for medical procedures in blood banks are events that may induce nanochanges in the membrane of red blood cells (RBCs). Alterations, such as the formation of pores and vesicles, reduce flexibility and compromise the overall erythrocyte integrity. This review discusses the alterations on erythrocytic lipid membrane bilayer through their characterization by confocal scanning microscopy, Raman, scanning electron microscopy, and atomic force microscopy techniques. The interrelated experimental results may address and shed light on the correlation of biomechanical and biochemical transformations induced in the membrane and cytoskeleton of stored and gamma-irradiated RBC. To highlight the main advantages of combining these experimental techniques simultaneously or sequentially, we discuss how those outcomes observed at micro- and nanoscale cell levels are useful as biomarkers of cell aging and storage damage. |
| Databáze: | OpenAIRE |
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