Magnetic particle transport through organogel – an application to DNA extraction –
| Autor: | Hiroki Kuyama, Tetsuo Ohashi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Materials science Magnetic Phenomena Solid Phase Extraction 010401 analytical chemistry Biophysics Methylphenylsilicone DNA Cell Biology Magnetic particle inspection Solid material 01 natural sciences Biochemistry DNA extraction 0104 chemical sciences Solvent 03 medical and health sciences Chemical engineering Magnetic nanoparticles Sample preparation Solid phase extraction Molecular Biology 030304 developmental biology |
| Zdroj: | Analytical Biochemistry. 611:113932 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/j.ab.2020.113932 |
| Popis: | An observation that magnetic particles are transported through organogel encouraged us to investigate its feasibility of liquid-phase displacement in DNA extraction using magnetic particles. Organogel for this study was prepared from a gelator, 12-hydroxystearic acid (12-HSA), and an apolar solvent, methylphenylsilicone oil. The organogel is a gel-like solid material with hydrophobic and elastic properties. These properties, hydrophobicity, and elasticity were demonstrated to be advantageous for liquid compartmentalization and efficient liquid-phase displacement. The extracted DNA with using the organogel device was successfully detected off-chip by conventional real-time PCR. |
| Databáze: | OpenAIRE |
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