Decoding the Nonvolatile Sensometabolome of Orange Juice (Citrus sinensis)
| Autor: | Andreas Dunkel, Oliver Frank, Anneke Glabasnia, Thomas Hofmann |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Limonins Male 0106 biological sciences Limonin Orange (colour) Fractionation Limonoid 01 natural sciences Flavones Mass Spectrometry chemistry.chemical_compound Glucosides medicine Humans Food science Chromatography High Pressure Liquid chemistry.chemical_classification Orange juice Molecular Structure 010401 analytical chemistry General Chemistry Bitter taste 0104 chemical sciences Flavoring Agents Fruit and Vegetable Juices chemistry Taste Female General Agricultural and Biological Sciences Citrus × sinensis Citrus sinensis 010606 plant biology & botany medicine.drug |
| Zdroj: | Journal of Agricultural and Food Chemistry. 66:2354-2369 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.7b06142 |
| Popis: | Activity-guided fractionation in combination with the taste dilution analysis, followed by liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance experiments, led to the identification of 10 polymethoxylated flavones (PMFs), 6 limonoid glucosides, and 2 limonoid aglycones as the key bitterns of orange juice. Quantitative studies and calculation of dose-over-threshold factors, followed by taste re-engineering, demonstrated for the first time 25 sensometabolites to be sufficient to reconstruct the typical taste profile of orange juices and indicated that not a single compound can be considered a suitable marker for juice bitterness. Intriguingly, the taste percept of orange juice seems to be created by a rather complex interplay of limonin, limonoid glucosides, PMFs, organic acids, and sugars. For the first time, sub-threshold concentrations of PMFs were shown to enhance the perceived bitterness of limonoids. Moreover, the influence of sugars on the perceived bitterness of limonoids and PMFs in orange juice relevant concentration ranges was quantitatively elucidated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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