Involvement of HLA-DR+ Large Granular Lymphocytes in the Induction of Tumor Necrosis Factor by Mycobacterium avium-intracellulare Complex
| Autor: | Herman Friedman, D. Kay Blanchard, Julie Y. Djeu, M. Beatriz Michelini‐Norris |
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| Rok vydání: | 1991 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Time Factors Lymphocyte medicine.medical_treatment Immunology Population CD2 Antigens chemical and pharmacologic phenomena Cell Separation Receptors Fc Biology CD16 Peripheral blood mononuclear cell Lymphocyte Depletion Monocytes medicine Humans Immunology and Allergy Receptors Immunologic education Mycobacterium avium-intracellulare Infection education.field_of_study Lymphokine-activated killer cell Tumor Necrosis Factor-alpha Receptors IgG HLA-DR Antigens Cell Biology Mycobacterium avium Complex Antigens Differentiation Molecular biology Lymphocyte Subsets Killer Cells Natural medicine.anatomical_structure Cytokine Tumor necrosis factor alpha CD8 |
| Zdroj: | Journal of Leukocyte Biology. 50:529-538 |
| ISSN: | 1938-3673 0741-5400 |
| DOI: | 10.1002/jlb.50.6.529 |
| Popis: | This study shows that normal human large granular lymphocytes (LGL) secrete tumor necrosis factor (TNF) in response to Mycobacterium avium-intracellulare complex (MAI). Percoll density gradient fractionation of peripheral mononuclear cells showed TNF activity in the fractions corresponding to LGL and not T cells, even when 5% monocytes were added to the T lymphocytes for accessory function. TNF release was not abrogated by treatment of the crude LGL preparations with anti-Leu M3. -CD4. and -CD8 antibodies (Ab) plus complement (C), but was abrogated by anti-CD16 and -CD2 Ab, as expected. Interestingly, anti-HLA-DR monoclonal antibody (mAb) treatment significantly diminished TNF activity from LGL, but maintained natural killer (NK) cell function unmodified as opposed to CD2 - and CD16- cell depletion. Panning studies demonstrated that TNF secretion upon MAI stimulation resided only in the HLA-DR+ LGL and not the DR LGL population. These results indicate that normal fresh HLA-DR+ LGL, as well as monocytes, are also responsible for rapid TNF secretion during early MAI infection. These DR - cells appear to be distinct from those expressing NK function. |
| Databáze: | OpenAIRE |
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