Identification of chickpea ( Cicer arietinum ) in foods using a novel real-time polymerase chain reaction detection method

Autor: Shinya Kimata, Tomoko Kobayashi, Junichi Mano, Takumi Ishigaki, Kazunari Kondo, Kazumi Kitta, Tomoko Nishimaki-Mogami, Reona Takabatake, Masahiro Kishine, Uki Fujii, Keisuke Soga, Kosuke Nakamura, Hiroshi Kawakami
Rok vydání: 2018
Předmět:
Zdroj: SC30201812190004
NARO成果DBd
ISSN: 0889-1575
DOI: 10.1016/j.jfca.2018.04.011
Popis: A novel real-time polymerase chain reaction (PCR) method for detecting chickpea was developed. From homologous sequences of 9-cis-epoxycarotenoid dioxygenase gene (NCED) among leguminous species, chickpea’s NCED was cloned, and the Southern-blot analysis showed that NCED is a single copy gene in a haploid genome. Its coding sequences at the 5′ terminus were found unique to chickpea and conserved among various chickpea varieties. Developed real-time PCR method targeting the unique sequences was specific to chickpea and had detection limit of approximately 30 copies per a reaction, and applicable for qualitative detection of chickpea in various forms of food products including dried, powdered, retort-packed, canned, fermented and pasted. Our results showed that the developed method enables identification of chickpea in foods.
Databáze: OpenAIRE
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