Ectopic expression of UGT84A2 delayed flowering by indole-3-butyric acid-mediated transcriptional repression of ARF6 and ARF8 genes in Arabidopsis
| Autor: | Shang-Hui Jin, Bing-Kai Hou, Pan Li, Yan-Jie Li, Xiao-Yi Jiang, G. Zhang |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Indoles Arabidopsis Plant Science Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Auxin Gene Expression Regulation Plant Glycosyltransferase Leafy Gene chemistry.chemical_classification Auxin homeostasis Arabidopsis Proteins fungi food and beverages General Medicine Indole-3-butyric acid biology.organism_classification Molecular biology DNA-Binding Proteins 030104 developmental biology chemistry Glucosyltransferases biology.protein Ectopic expression Agronomy and Crop Science 010606 plant biology & botany Transcription Factors |
| Zdroj: | Plant cell reports. 36(12) |
| ISSN: | 1432-203X |
| Popis: | Ectopic expression of auxin glycosyltransferase UGT84A2 in Arabidopsis can delay flowering through increased indole-3-butyric acid and suppressed transcription of ARF6, ARF8 and flowering-related genes FT, SOC1, AP1 and LFY. Auxins are critical regulators for plant growth and developmental processes. Auxin homeostasis is thus an important issue for plant biology. Here, we identified an indole-3-butyric acid (IBA)-specific glycosyltransferase, UGT84A2, and characterized its role in Arabidopsis flowering development. UGT84A2 could catalyze the glycosylation of IBA, but not indole-3-acetic acid (IAA). UGT84A2 transcription expression was clearly induced by IBA. When ectopically expressing in Arabidopsis, UGT84A2 caused obvious delay in flowering. Correspondingly, the increase of IBA level, the down-regulation of AUXIN RESPONSE FACTOR 6 (ARF6) and ARF8, and the down-regulation of flowering-related genes such as FLOWERING LOCUS T (FT), SUPPRESSOR OF OVEREXPRESSION OF CO1(SOC1), APETALA1 (AP1), and LEAFY(LFY) were observed in transgenic plants. When exogenously applying IBA to wild-type plants, the late flowering phenotype, the down-regulation of ARF6, ARF8 and flowering-related genes recurred. We examined the arf6arf8 double mutants and found that the expression of flowering-related genes was also substantially decreased in these mutants. Together, our results suggest that glycosyltransferase UGT84A2 may be involved in flowering regulation through indole-3-butyric acid-mediated transcriptional repression of ARF6, ARF8 and downstream flowering pathway genes. |
| Databáze: | OpenAIRE |
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