Polyoma virus-specific RNA synthesis in an inducible line of polyoma virus-transformed rat cells
| Autor: | H Manor, R Kamen |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
DNA Replication
Cytoplasm Transcription Genetic DNA polymerase Immunology RNA-dependent RNA polymerase Origin of replication Microbiology DNA polymerase delta Cell Line Mitomycins chemistry.chemical_compound Transcription (biology) Virology Animals RNA Messenger Cell Nucleus biology RNA Nuclease protection assay Cell Transformation Viral Molecular biology Rats Cell Transformation Neoplastic chemistry Insect Science DNA Viral biology.protein RNA Viral Floxuridine Polyomavirus DNA Research Article |
| Zdroj: | Journal of Virology. 25:719-729 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.25.3.719-729.1978 |
| Popis: | Viral RNA present in the inducible LPT clone 1A of polyoma virus-transformed rat cells was characterized before and after mitomycin C induction by hybridization with 32P-labeled separated E and L strands of polyoma viral DNA restriction endonuclease fragments. In clone 1A cells maintained under normal growth conditions, the cytoplasm contained a transcript of the E-strand DNA from the "early" region similar to that previously identified in lytically infected cells, as well as minor quantities of RNA complementary to less than one-half of the L- and the E-strand DNA from the "late" region. Nuclei of normally growing cells contained the same species found in the cytoplasm, as well as an additional abundant RNA complementary to one-half of the L-strand DNA of the late region. No significant changes occurred in the cytoplasmic viral RNA after mitomycin C treatment before the onset of viral DNA replication, but the concentration of the nuclear L-strand DNA transcript diminished. After the onset of viral DNA replication after mitomycin C treatment, transcripts of virtually the entire L-strand DNA were found in the nuclei, and a 10-fold increase was observed in the abundance of RNA transcribed from the E strand of the early region. In the cytoplasm, the abundance of the early RNA increased about 25-fold and late RNA complementary to the L-strand DNA of the late region was found in a similar quantity. The synthesis of both the early and the late RNA species was inhibited if viral DNA replication was blocked with 5-fluorodeoxyuridine. We conclude that the induction of viral DNA replication in LPT cells is not determined at the level of mRNA synthesis. |
| Databáze: | OpenAIRE |
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