High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds
| Autor: | Kyle B. Kener, Jeffery S. Tessem, Devin J. Munk, Chad R. Hancock |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030109 nutrition & dietetics General Immunology and Microbiology Cellular respiration Respiration General Chemical Engineering General Neuroscience chemistry.chemical_element Mitochondrion Carbohydrate metabolism Oxygen General Biochemistry Genetics and Molecular Biology Mitochondria 03 medical and health sciences Respirometry Cellular Biology Oxygen Consumption chemistry Biochemistry Insulin-Secreting Cells Humans Beta cell Beta (finance) |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/57053-v |
| Popis: | High-resolution respirometry allows for the measurement of oxygen consumption of isolated mitochondria, cells and tissues. Beta cells play a critical role in the body by controlling blood glucose levels through insulin secretion in response to elevated glucose concentrations. Insulin secretion is controlled by glucose metabolism and mitochondrial respiration. Therefore, measuring intact beta cell respiration is essential to be able to improve beta cell function as a treatment for diabetes. Using intact 832/13 INS-1 derived beta cells we can measure the effect of increasing glucose concentration on cellular respiration. This protocol allows us to measure beta cell respiration in the presence or absence of various compounds, allowing one to determine the effect of given compounds on intact cell respiration. Here we demonstrate the effect of two naturally occurring compounds, monomeric epicatechin and curcumin, on beta cell respiration under the presence of low (2.5 mM) or high glucose (16.7 mM) conditions. This technique can be used to determine the effect of various compounds on intact beta cell respiration in the presence of differing glucose concentrations. |
| Databáze: | OpenAIRE |
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