Electrooptics Studies of Escherichia coli Electropulsation: Orientation, Permeabilization, and Gene Transfer
| Autor: | Frédéric Rodriguez, Justin Teissié, N. Eynard, J. Trotard |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cell Membrane Permeability
Light Kinetics Biophysics Analytical chemistry Biology Transfection medicine.disease_cause Plasmid Nephelometry and Turbidimetry Escherichia coli medicine Microscopy Video Pulse (signal processing) Electroporation Gene Transfer Techniques Pulse duration DNA biology.organism_classification Transformation (genetics) Bacteria Plasmids Research Article |
| Zdroj: | Biophysical Journal. 75:2587-2596 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/s0006-3495(98)77704-5 |
| Popis: | Fast optical transient signals are suitable approaches to the investigation of the behavior of bacteria during an electric pulse. In a previous work, by a dual approach taking advantage of a video method and a fast kinetic study of the light transmitted across a cell suspension, we showed that a field-induced orientation phenomenon was affecting the rod-shaped bacteria during the pulse (Eynard et al., 1992. Eur. J. Biochem. 209:431-436). In the present work, time courses of electro-induced responses of bacteria during a single square-wave pulse are analyzed. Observations of both the orientation step and the permeabilization process are relevant. These two steps are affected by the addition of DNA. They both obey to a first-order kinetic. The conclusion of this work is that Escherichia coli permeabilization and transformation are multistep processes: orientation (step 1) is followed by an envelope alteration (step 2), all steps being affected by plasmid addition. In the case of E. coli, a rod-shaped bacteria, the orientation process (step 1) brings the cell parallel to the field direction. The pulse duration must be longer than the orientation characteristic time (approximately 1 ms) to trigger an effective permeabilization and its associated events. The permeabilization process (step 2) is associated with a field-induced dipole effect. |
| Databáze: | OpenAIRE |
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