New strategy to surface functionalization of polymeric nanoparticles: one-pot synthesis of scFv anti-LDL(−)-functionalized nanocapsules
| Autor: | Marcela Frota Cavalcante, Dulcinéia Saes Parra Abdalla, Silvia Stanisçuaski Guterres, Adriana Raffin Pohlmann, Eduardo André Bender, Letícia Marques Colomé, Márcia Duarte Adorne |
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| Rok vydání: | 2014 |
| Předmět: |
Polymers
Chemistry Pharmaceutical One-pot synthesis LIPOPROTÉINAS LDL Pharmaceutical Science Nanoparticle Ligands Nanocapsules chemistry.chemical_compound Molecular recognition Catalytic Domain Lecithins Organic chemistry Pharmacology (medical) Particle Size Pharmacology Chitosan biology Chemistry Organic Chemistry Active site Combinatorial chemistry Lipoproteins LDL Oxidative Stress Metals Polycaprolactone biology.protein Nanoparticles Molecular Medicine Surface modification Nanomedicine Lipid Peroxidation Single-Chain Antibodies Biotechnology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1573-904X 0724-8741 |
| DOI: | 10.1007/s11095-014-1392-5 |
| Popis: | In general, the surface functionalization of polymeric nanoparticles is carried out by covalently bounding ligands to the nanoparticle surface. This process can cause a lack or decrease of the ligand specificity to its target receptor, besides the need of purification steps. We proposed a ligand-metal-chitosan-lecithin complex as a new strategy to functionalize the surface of biodegradable nanoparticles. One pot synthesis of scFv anti-LDL(−)-functionalized nanocapsules was carried out by self-assembly and interfacial reactions. Particle sizing techniques, lipid peroxidation and molecular recognition by enzyme linked immuno sorbent assays were carried out. The selected formulation had unimodal size distribution with mean diameter of about 130 nm. The metals in the complex did not enhance the oxidative stress, and the scFv anti-LDL(−)-functionalized nanocapsules recognized LDL(−) and did not react with native LDL indicating the maintenance of the active site of the fragment. The one pot synthesis, using the ligand-metal-chitosan-lecithin complex to functionalize the surface of the biodegradable nanocapsules, maintained the active site of the antibody fragment making the device interesting for applications in nanomedicine. |
| Databáze: | OpenAIRE |
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