Calcium phosphate mineralization through homogenous enzymatic catalysis: Investigation of the early stages
| Autor: | Christophe Méthivier, Dalil Brouri, K. El Kirat, Jessem Landoulsi, F. Oudet, Clément Guibert, L. Valentin, Elodie Colaço |
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| Přispěvatelé: | Biomécanique et Bioingénierie (BMBI), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Réactivité de Surface (LRS), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université de Technologie de Compiègne (UTC) |
| Rok vydání: | 2019 |
| Předmět: |
Mineralized tissues
Calcium Phosphates Surface Properties chemistry.chemical_element 02 engineering and technology engineering.material Calcium 010402 general chemistry 01 natural sciences law.invention Biomaterials Colloid and Surface Chemistry law [CHIM]Chemical Sciences Animals Brushite Crystallization Intestinal Mucosa Particle Size Chemistry Substrate (chemistry) Mineralization (soil science) 021001 nanoscience & nanotechnology Alkaline Phosphatase 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Chemical engineering Whitlockite engineering Biocatalysis Cattle 0210 nano-technology Biomineralization |
| Zdroj: | Journal of Colloid and Interface Science Journal of Colloid and Interface Science, Elsevier, 2020, 565, pp.43-54. ⟨10.1016/j.jcis.2019.12.097⟩ |
| ISSN: | 1095-7103 0021-9797 |
| Popis: | Hypothesis The crystallization of calcium phosphate (CaP) is a ubiquitous process that occurs in several mineralized tissues and involves a variety of biochemical and chemical reactions. This issue has been hitherto continuously studied from supersaturated solutions (chemical procedure), i.e. by adding calcium and orthophosphate ions in a homogenous phase. Yet, both in vivo and in vitro investigations have clearly shown the implication of enzymes, namely alkaline phosphatase (ALP), to initiate the mineralization process by generating orthophosphate ions. Experiments We report a thorough investigation on the mechanism of enzyme-induced mineralization in homogenous phase (enzymatic procedure). For this purpose, ALP is introduced in Ca2+/Mg2+-containing solution (pH = 7.4; 37 °C), and its activity modulated by the concentration of its substrate. Findings Results show that after 24 h of mineralization both chemical and enzymatic procedures lead to the formation of well-crystalline hydroxyapatite nano-objects, however with noticeable impact on their shape and dimensions. Remarkably enough, by combining in situ monitoring and ex situ characterizations, we identify several intermediate phases, including amorphous phase, dicalcium phosphate dehydrate phase (DCPD or brushite) and Whitlockite (WH). Besides, mineralized nano-objects with a core-shell structure is observed, and hydroxyapatite platelets are shown to grow on the surface of their shell. |
| Databáze: | OpenAIRE |
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