BPSL1626 : reverse and structural vaccinology reveal a novel candidate for vaccine design against Burkholderia pseudomallei
| Autor: | Oscar Conchillo-Solé, Marina Cretich, Martino Bolognesi, Arnone Nithichanon, Claudio Peri, Louise J. Gourlay, Riccardo Capelli, Daniel Yero, Marcella Chiari, Giorgio Colombo, Ganjana Lertmemongkolchai, Xavier Daura, Paola Gagni, Riccardo Villa |
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| Rok vydání: | 2021 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
0301 basic medicine crystal structure Immunogen Melioidosis Burkholderia 030231 tropical medicine Immunology Context (language use) type I fimbrial subunit Article Epitope 03 medical and health sciences reverse vaccinology 0302 clinical medicine Antigen Drug Discovery medicine Immunology and Allergy Type I fimbrial subunit in silico epitope predictions biology Burkholderia pseudomallei Crystal structure Reverse vaccinology BPSL1626 antigen biology.organism_classification medicine.disease Virology 3. Good health 030104 developmental biology ddc:540 melioidosis lcsh:RC581-607 In silico epitope predictions |
| Zdroj: | Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) Recercat. Dipósit de la Recerca de Catalunya instname Antibodies Volume 7 Issue 3 Antibodies, Vol 7, Iss 3, p 26 (2018) 'Antibodies ', vol: 7, pages: 26-1-26-14 (2018) Antibodies 7(3), 26-(2018). doi:10.3390/antib7030026 Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| ISSN: | 2073-4468 |
| DOI: | 10.3390/antib7030026 |
| Popis: | Due to significant advances in computational biology, protein prediction, together with antigen and epitope design, have rapidly moved from conventional methods, based on experimental approaches, to in silico-based bioinformatics methods. In this context, we report a reverse vaccinology study that identified a panel of 104 candidate antigens from the Gram-negative bacterial pathogen Burkholderia pseudomallei, which is responsible for the disease melioidosis. B. pseudomallei can cause fatal sepsis in endemic populations in the tropical regions of the world and treatment with antibiotics is mostly ineffective. With the aim of identifying potential vaccine candidates, we report the experimental validation of predicted antigen and type I fimbrial subunit, BPSL1626, which we show is able to recognize and bind human antibodies from the sera of Burkholderia infected patients and to stimulate T-lymphocytes in vitro. The prerequisite for a melioidosis vaccine, in fact, is that both antibody- and cell-mediated immune responses must be triggered. In order to reveal potential antigenic regions of the protein that may aid immunogen re-design, we also report the crystal structure of BPSL1626 at 1.9 Å resolution on which structure-based epitope predictions were based. Overall, our data suggest that BPSL1626 and three epitope regions here-identified can represent viable candidates as potential antigenic molecules. |
| Databáze: | OpenAIRE |
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