Metabolite profiling in rat urine by liquid chromatography/electrospray ion trap mass spectrometry. Application to the study of heavy metal toxicity
| Autor: | Christophe Junot, Eric Ezan, Alexandra Lafaye, Jean-Claude Tabet, P. Fritsch, Béatrice Ramounet-Le Gall |
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| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
Electrospray Spectrometry Mass Electrospray Ionization Chromatography Chemistry Organic Chemistry Glycoside Urine DNA Vitamins Mass spectrometry Tandem mass spectrometry Peptide Mapping Analytical Chemistry Ion Rats Metals Heavy Metabolome Animals Uranium Ion trap Amino Acids Spectroscopy Chromatography High Pressure Liquid Cadmium |
| Zdroj: | Rapid communications in mass spectrometry : RCM. 17(22) |
| ISSN: | 0951-4198 |
| Popis: | This work reports the use of reverse-phase liquid chromatography coupled to electrospray ion trap (QIT) mass spectrometry for the analysis of the metabolome in rat urine. An injection of 20 μL of urine into the chromatographic system is followed by a slow gradient elution and mass spectrometric detection in the scanning mode from m/z 100–1000 in both positive and negative modes. Over a time scale of 90 min, 30 and 20 resolved peaks were observed in the positive and the negative modes, respectively, corresponding to the presence of a few hundred m/z ratios. By using a QIT analyzer, data-dependent tandem mass spectrometry of selected m/z ratios identified several compounds in rat urine and characterized various chemical families, including carboxylic acids, amines, sulfated compounds, glucuronides and glycosides, by the observation of characteristic fragment ions or neutral losses. The method has been applied to the investigation of the chronic toxicity of heavy metals in rat urine. A few tens of m/z ratios, differing in intensity more than threefold from control values, were observed in both positive and negative modes. The time variations for some selected ions suggest that LC/ESI-MS could allow selective characterization of biomarkers in response to specific toxic compounds. Copyright © 2003 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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