Cell autonomous roles for AP-2α in lens vesicle separation and maintenance of the lens epithelial cell phenotype
| Autor: | Shelley Sullivan, Ales Cvekl, Trevor Williams, Paula Deschamps, Ruth Ashery-Padan, Judith A. West-Mays, Ryan Wong, Giuseppe F. Pontoriero, Jiri Zavadil, Ying Yang |
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| Rok vydání: | 2008 |
| Předmět: |
PAX6 Transcription Factor
Mutant Biology Eye Article law.invention Mice law Lens Crystalline medicine Animals Paired Box Transcription Factors Lens placode Eye Proteins Oligonucleotide Array Sequence Analysis Corneal epithelium Homeodomain Proteins Forkhead Transcription Factors Cadherins Molecular biology Phenotype Mice Mutant Strains Repressor Proteins Lens (optics) medicine.anatomical_structure Transcription Factor AP-2 Optic cup (embryology) Eye development PAX6 Transcription Factors Developmental Biology |
| Zdroj: | Developmental Dynamics. 237:602-617 |
| ISSN: | 1097-0177 1058-8388 |
| DOI: | 10.1002/dvdy.21445 |
| Popis: | In this study, we have created a conditional deletion of AP-2alpha in the developing mouse lens (Le-AP-2alpha mutants) to determine the cell-autonomous requirement(s) for AP-2alpha in lens development. Embryonic and adult Le-AP-2alpha mutants exhibited defects confined to lens placode derivatives, including a persistent adhesion of the lens to the overlying corneal epithelium (or lens stalk). Expression of known regulators of lens vesicle separation, including Pax6, Pitx3, and Foxe3 was observed in the Le-AP-2alpha mutant lens demonstrating that these genes do not lie directly downstream of AP-2alpha. Unlike germ-line mutants, Le-AP-2alpha mutants did not exhibit defects in the optic cup, further defining the tissue specific role(s) for AP-2alpha in eye development. Finally, comparative microarray analysis of lenses from the Le-AP-2alpha mutants vs. wild-type littermates revealed differential expression of 415 mRNAs, including reduced expression of genes important for maintaining the lens epithelial cell phenotype, such as E-cadherin. |
| Databáze: | OpenAIRE |
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