Transport of serotonin from the rat jejunal lumen into mesenteric veins in vivo
| Autor: | Håkan Ahlman, A. Dahlström, K. O. Grönstad, I. Larsson |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
Serotonin medicine.medical_specialty Physiology Biological Transport Active Lumen (anatomy) Biology Mesenteric Vein Jejunum Mesenteric Veins Internal medicine medicine Animals Platelet Intestinal Mucosa Whole blood Rats Inbred Strains Venous blood Small intestine Rats Endocrinology medicine.anatomical_structure Intestinal Absorption Biochemistry Carrier Proteins |
| Zdroj: | Acta Physiologica Scandinavica. 138:403-407 |
| ISSN: | 1365-201X 0001-6772 |
| DOI: | 10.1111/j.1748-1716.1990.tb08863.x |
| Popis: | The transport of labelled (hot) and non-labelled (cold) serotonin (5-HT) into the mesenteric venous circulation was studied after instillation of test solutions into an isolated jejunal loop of anaesthetized rats. After instillation of [3H]H2O and [14C]5-HT there was an almost parallel appearance of the isotopes in mesenteric venous blood. After instillation of 5-HT a marked early increase of the total amounts of cold 5-HT was observed in mesenteric veins compared with animals instilled with saline only. In a third type of experiment the label was detected in mesenteric venous whole blood after instillation of [3H]5-HT into the gut lumen. After hydrolysis of blood cells and protein precipitation the samples were fractionated and determined for 5-HT and metabolites. Only 5-HT was detected in these fractions. The label was present within 5-HT peaks in three out of eight animals. The experiments indicate rapid transport of 5-HT (or metabolites) across the rat jejunal mucosa. These substances may be bound to a binding protein in platelets since the isotope was detected in whole blood but more seldom in supernatants after hydrolysis and precipitation. |
| Databáze: | OpenAIRE |
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