Administration of PLP139–151 Primes T Cells Distinct from Those Spontaneously Responsive In Vitro to This Antigen
| Autor: | Peter van den Elzen, Chiara Agrati, Gabriele Di Sante, Francesco Ria, Francesca Aloisi, Eli E. Sercarz, Chiara Nicolò, Romina Penitente |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Encephalomyelitis
Autoimmune Experimental Proteolipid protein 1 T-Lymphocytes Immunology Receptors Antigen T-Cell Autoimmunity chemical and pharmacologic phenomena Spleen In Vitro Techniques Gene Rearrangement T-Lymphocyte Lymphocyte Activation Autoantigens Mice Antigen Settore MED/04 - PATOLOGIA GENERALE Immune Tolerance medicine Animals Immunology and Allergy Myelin Proteolipid Protein Antigen Presentation EAE business.industry T-cell receptor Experimental autoimmune encephalomyelitis medicine.disease Peptide Fragments In vitro medicine.anatomical_structure Immunization Female Lymph business TCR |
| Zdroj: | ResearcherID |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.180.10.6611 |
| Popis: | We examined the TCR repertoire used by naive SJL mice in their in vitro spontaneous response to proteolipid protein (PLP) 139–151 by Vβ-Jβ spectratyping and compared it to that used after immunization with the peptide. T cells from immunized mice use the public rearrangement Vβ10-Jβ1.1, but naive mice do not; in contrast, TCR CDR3-β rearrangements of Vβ18-Jβ1.2 and Vβ19-Jβ1.2 consistently are associated with the spontaneous response. T cells involved in spontaneous and induced responses can each recognize PLP139–151 presented in vivo, but its s.c. administration has different consequences for the two repertoires. Four days after immunization, T cells associated with spontaneous responsiveness appear in the draining lymph nodes but disappear by day 10 and never appear elsewhere. Simultaneously, Vβ10-Jβ1.1 T cells are likewise activated in the lymph nodes by day 4 and spread to the spleen by day 10. Eight- to 10-wk-old naive mice use a narrower repertoire of TCRs than do immunized age-matched mice. Induced Vβ10-Jβ1.1 T cells home to the CNS during experimental autoimmune encephalomyelitis, whereas we failed to detect Vβ18-Jβ1.2 and Vβ19-Jβ1.2 TCR rearrangements in the CNS. Thus, we observe that administration of PLP139–151 primes a T cell repertoire distinct from the one responsible for spontaneous responsiveness. This “immunized” repertoire substitutes for the naive one and becomes dominant at the time of disease onset. |
| Databáze: | OpenAIRE |
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