Altered Expression of a Chloroplast Protein Affects the Outcome of Virus and Nematode Infection
| Autor: | Perrodin-Njoku E, Carlew Ts, Tessa M. Burch-Smith, John Hollis Rice, Tarek Hewezi, Elena E. Ganusova, Patel A |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology viruses Potyvirus Arabidopsis Nicotiana benthamiana 01 natural sciences Virus Chloroplast Proteins 03 medical and health sciences Gene Expression Regulation Plant Plant virus Tobacco Tobacco mosaic virus medicine Animals Turnip mosaic virus Gene Silencing Tylenchoidea Plant Diseases biology Arabidopsis Proteins fungi Plasmodesmata food and beverages General Medicine Plants Genetically Modified biology.organism_classification medicine.disease Virology Cell biology Tobacco Mosaic Virus Protein Transport RNA silencing 030104 developmental biology Nematode infection Host-Pathogen Interactions Agronomy and Crop Science Heterodera schachtii RNA Helicases 010606 plant biology & botany |
| Zdroj: | Molecular Plant-Microbe Interactions®. 30:478-488 |
| ISSN: | 1943-7706 0894-0282 |
| Popis: | The chloroplast-resident RNA helicase ISE2 (INCREASED SIZE EXCLUSION LIMIT2) can modulate the formation and distribution of plasmodesmata and intercellular trafficking. We have determined that ISE2 expression is induced by viral infection. Therefore, the responses of Nicotiana benthamiana plants with varying levels of ISE2 expression to infection by Tobacco mosaic virus and Turnip mosaic virus were examined. Surprisingly, increased or decreased ISE2 expression led to faster viral systemic spread and, in some cases, enhanced systemic necrosis. The contributions of RNA silencing and hormone-mediated immune responses to the increased viral susceptibility of these plants were assessed. In addition, Arabidopsis thaliana plants with increased ISE2 expression were found to be more susceptible to infection by the beet cyst nematode Heterodera schachtii. Our analyses provide intriguing insights into unexpected functional roles of a chloroplast protein in mediating plant-pathogen interactions. The possible roles of plasmodesmata in determining the outcomes of these interactions are also discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|