Latex C-serum fromHevea brasiliensisinduces non-apoptotic cell death in hepatocellular carcinoma cell line (HepG2)
| Autor: | K. L. Yang, Ming Thong Ong, K. L. Lam, E. Sunderasan |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Programmed cell death
Carcinoma Hepatocellular Latex Cell Survival Biology Cell Line Cell Line Tumor Carcinoma medicine Humans Cytotoxic T cell MTT assay Viability assay Cell Proliferation bcl-2-Associated X Protein Cell Death Liver Neoplasms Hep G2 Cells Original Articles Cell Biology General Medicine medicine.disease biology.organism_classification Antineoplastic Agents Phytogenic Molecular biology Gene Expression Regulation Neoplastic Proto-Oncogene Proteins c-bcl-2 Cell culture Hevea DNA fragmentation Hevea brasiliensis |
| Zdroj: | Cell Proliferation. 45:577-585 |
| ISSN: | 0960-7722 |
| DOI: | 10.1111/j.1365-2184.2012.00841.x |
| Popis: | OBJECTIVES: Latex from Hevea brasiliensis (natural rubber tree primarily cultivated for its rubber particles) has no known primary metabolic function, although its biological role is as a plant defence system. The present study has evaluated specific anti‐proliferative effects of latex whole C‐serum and its subfractions, on human cancer cell lines. MATERIALS AND METHODS: Cell viability assay using MTT, DNA fragmentation assay and real‐time PCR were used to evaluate the cytotoxic effects of latex whole C‐serum and its subfractions on the cell lines. RESULTS: MTT assay revealed very low LC (50) values, 2.0 and 280 ng/ml, for DCS and DCP treatments, respectively. DCS was proven to be more potent compared to DCP, in conferring specific anti‐proliferative effects on the cancer cell lines. The study also indicated that anti‐proliferative activity of pre‐heated C‐serum fractions diminished significantly. CONCLUSION: Although noteworthy cell death was reported, DNA fragmentation assay and real‐time PCR confirmed that that induced by latex C‐serum subfractions was not promoted via the classical apoptotic signalling pathway. |
| Databáze: | OpenAIRE |
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