Anti-cancerous efficacy and pharmacokinetics of 6-mercaptopurine loaded chitosan nanoparticles
| Autor: | Syamantak Mani Tripathi, C Manohara, R. G. S. V. Prasad, A.R. Phani, Jagadeesh S. Sanganal, G. Prem Kumar, S. Amaresha, HL Raghavendra, P.B. Janardhana, K. B. Swamy |
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| Rok vydání: | 2015 |
| Předmět: |
Drug
media_common.quotation_subject Biological Availability Antineoplastic Agents Apoptosis Pharmacology Chitosan chemistry.chemical_compound Drug Delivery Systems Pharmacokinetics Cell Line Tumor Humans MTT assay Particle Size media_common Drug Carriers Mercaptopurine Cell Cycle In vitro Bioavailability chemistry Delayed-Action Preparations MCF-7 Cells Nanoparticles Drug carrier |
| Zdroj: | Pharmacological Research. 100:47-57 |
| ISSN: | 1043-6618 |
| DOI: | 10.1016/j.phrs.2015.07.025 |
| Popis: | 6-Mercaptopurine is a cytotoxic and immunosuppressant drug. The use of this drug is limited due to its poor bioavailability and short plasma half-life. In order to nullify these drawbacks, 6-mercaptopurine-chitosan nanoparticles (6-MP-CNPs) were prepared and evaluated to study the influence of preparation conditions on the physicochemical properties by using DLS, SEM, XRD and FTIR. The in vitro drug release profile at pH 4.8 and 7.4 revealed sustained release patterns for a period of 2 days. The nanoformulations showed enhanced in vitro anti-cancer activities (MTT assay, apoptosis assay, cell cycle arrest and ROS indices) on HT-1080 and MCF-7 cells. In vivo pharmacokinetics profiles of 6-MP-CNPs showed improved bioavailability. Thus, the results of the present study revealed that, the prepared 6-MP-CNPs have a significant role in increasing anti-cancer efficacy, bioavailability and in vivo pharmacokinetics profiles. |
| Databáze: | OpenAIRE |
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