Candida albicans isolated from urine: Phenotypic and molecular identification, virulence factors and antifungal susceptibility
| Autor: | Renata Pires de Araújo, Alexeia Barufatti Grisolia, Bruna de Paula Bicudo, Adriana Araújo de Almeida-Apolonio, Luana Mireli Carbonera Rodrigues, Laura Wiebusch, Danielly Beraldo dos Santos Silva, Danielle Ferreira Lonchiati, Kelly Mari Pires de Oliveira |
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| Přispěvatelé: | Fundacao Univ Fed Grande Dourados, Universidade Federal de Mato Grosso do Sul (UFMS), Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
lcsh:Arctic medicine. Tropical medicine Itraconazole lcsh:RC955-962 030106 microbiology Virulence Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Microbiology 03 medical and health sciences Minimum inhibitory concentration Amphotericin B medicine Candida albicans lcsh:QH301-705.5 Voriconazole Urinary tract Broth microdilution Candidiasis biology.organism_classification lcsh:Biology (General) Infection Antifungal susceptibility Fluconazole medicine.drug |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 7, Pp 624-628 (2017) |
| Popis: | Made available in DSpace on 2019-10-03T18:18:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-07-01 Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia de Mato Grosso do Sul Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Objective: To isolate Candida albicans (C. albicans) from the urine of hospitalized patients and assess the virulence factors and antifungal susceptibility profiles of the isolates. Methods: Yeasts were identified using the chromogenic medium CHROMagar (TM), the VITEK (R) 2 system, hypertonic Sabouraud broth, tobacco agar, polymerase chain reaction, and DNA sequencing. The evaluated virulence factors were proteinase production, phospholipase production, and biofilm production on polystyrene. The broth micro-dilution technique was used to determine the minimum inhibitory concentration. Results: All yeasts isolated from urine were identified as C. albicans using both classical and molecular methods. Although 91.3% of the isolates showed no phospholipase activity, 56.5% showed strong proteinase activity and 91.7% produced biofilm. All microorganisms were sensitive to fluconazole, voriconazole and amphotericin B, but 56.5% of the yeasts showed resistance to itraconazole. Conclusions: C. albicans isolates from urine have a high capacity for virulence and can be associated with infectious processes. Furthermore, the high percentage of isolates resistant to itraconazole is important because this antifungal agent is commonly used to treat fungal infections in the hospital environment. Fundacao Univ Fed Grande Dourados, Fac Ciencias Biol & Ambientais, Rodovia Dourados Itahum,Km 12,Caixa Postal 533, BR-79804970 Dourados, MS, Brazil Univ Fed Mato Grosso do Sul, Fac Med, Campo Grande, MS, Brazil Fundacao Univ Fed Grande Dourados, Fac Ciencias Saude, Dourados, MS, Brazil Univ Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, SP, Brazil Univ Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, SP, Brazil Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia de Mato Grosso do Sul: 0068/12 Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia de Mato Grosso do Sul: SIAFEM: 020666 |
| Databáze: | OpenAIRE |
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