Vosaroxin induces mitochondrial dysfunction and apoptosis in cervical cancer HeLa cells: Involvement of AMPK/Sirt3/HIF-1 pathway
| Autor: | Chun-Zhi Yu, Xian-Li Zhao |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
SOD2 Uterine Cervical Neoplasms Antineoplastic Agents Apoptosis Mitochondrion Protein degradation AMP-Activated Protein Kinases Toxicology Vosaroxin HeLa 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Sirtuin 3 Humans Viability assay Naphthyridines RNA Small Interfering Protein kinase A biology Chemistry Superoxide Dismutase Aryl Hydrocarbon Receptor Nuclear Translocator General Medicine biology.organism_classification Hypoxia-Inducible Factor 1 alpha Subunit Mitochondria Thiazoles 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Female RNA Interference Lipid Peroxidation Reactive Oxygen Species HeLa Cells Signal Transduction |
| Zdroj: | Chemico-biological interactions. 290 |
| ISSN: | 1872-7786 |
| Popis: | Vosaroxin is a quinolone-derivative anticancer agent with inhibitory activity on type II DNA topoisomerases (TOP2). The aim of the present study was to investigate its cytotoxic effect and potential molecular mechanisms in human cervical cancer HeLa cells. Vosaroxin decreased cell viability and increased lactate dehydrogenase (LDH) release in a dose- and time-dependent manner in HeLa cells, but not in normal cervical epithelial cells. Vosaroxin also induced apoptosis and increased caspase-3 activity in HeLa cells. These effects were accompanied by increased mitochondrial reactive oxygen species (ROS) generation, lipid peroxidation, mitochondrial swelling and reduced ATP production. Western blot analysis showed that vosaroxin significantly reduced hypoxia-inducible factor 1α (HIF-1α) protein levels. However, it had no effect on HIF-1α protein degradation and HIF-1α mRNA levels. The results showed that vosaroxin inhibited the synthesis of HIF-1α protein and interfered with the dimerization of HIF-1α and aryl hydrocarbon receptor nuclear translocator (ARNT). In addition, vosaroxin stimulated mitochondrial enzyme activities and superoxide dismutase 2 (SOD2) deacetylation via activating (Sir2 like protein 3) Sirt3. More importantly, vosaroxin-induced inhibition on HIF-1α and its cytotoxic effects, as measured by cell viability, LDH release and apoptosis, were partially prevented by Sirt3 knockdown or the AMP-activated protein kinase (AMPK) inhibitor compound C. Overall, vosaroxin is demonstrated to be a chemotherapeutic agent targeting the Sirt3/HIF-1 pathway and could be beneficial for inducing cytotoxicity in human cervical cancer cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect