Important roles of Vilse in dendritic architecture and synaptic plasticity
Autor: | Ming-Yuan Min, Jin-Yu Lee, Mau-Sun Chang, Chih-Chen Fan, Ho-Ching Chang, Li-Jen Lee, Hsin-An Shih |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
Male
0301 basic medicine Transgene Morphogenesis Mice Transgenic Water maze Neurotransmission Hippocampal formation Synaptic Transmission Article WW domain 03 medical and health sciences Metaplasticity Animals Maze Learning CA1 Region Hippocampal Spatial Memory Mice Knockout Neuronal Plasticity Multidisciplinary biology Pyramidal Cells GTPase-Activating Proteins Dendrites Cell biology Mice Inbred C57BL 030104 developmental biology Synaptic plasticity biology.protein Female Genes Lethal |
Zdroj: | Scientific Reports |
ISSN: | 2045-2322 |
DOI: | 10.1038/srep45646 |
Popis: | Vilse/Arhgap39 is a Rho GTPase activating protein (RhoGAP) and utilizes its WW domain to regulate Rac/Cdc42-dependent morphogenesis in Drosophila and murine hippocampal neurons. However, the function of Vilse in mammalian dendrite architecture and synaptic plasticity remained unclear. In the present study, we aimed to explore the possible role of Vilse in dendritic structure and synaptic function in the brain. Homozygous knockout of Vilse resulted in premature embryonic lethality in mice. Changes in dendritic complexity and spine density were noticed in hippocampal neurons of Camk2a-Cre mediated forebrain-specific Vilse knockout (VilseΔ/Δ) mice. VilseΔ/Δ mice displayed impaired spatial memory in water maze and Y-maze tests. Electrical stimulation in hippocampal CA1 region revealed that the synaptic transmission and plasticity were defected in VilseΔ/Δ mice. Collectively, our results demonstrate that Vilse is essential for embryonic development and required for spatial memory. |
Databáze: | OpenAIRE |
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