Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system
| Autor: | Osami Kanagawa, Akihiro Hata, Michio Tomura, Kenji Ohtawa, Atsushi Miyawaki, Asako Sakaue-Sawano, Mitsuyo Takase-Utsugi, Yoshiko Mori |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Genetically modified mouse Cell division Hematopoietic System Cellular differentiation Transgene lcsh:Medicine Mice Transgenic Biology Resting Phase Cell Cycle Flow cytometry Mice Immune system medicine Animals lcsh:Science Cells Cultured Multidisciplinary medicine.diagnostic_test Cell Cycle lcsh:R G1 Phase Cell cycle Flow Cytometry Cell biology Haematopoiesis Immune System Female lcsh:Q Cell Division Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 9, p e73801 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and these correspond to quiescent G0 and cycling G1 cells, respectively. We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and immune responses. |
| Databáze: | OpenAIRE |
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