A bioprinted composite hydrogel with controlled shear stress on cells
| Autor: | Hamid Rashedi, Fatemeh Yazdian, Amirhossein Bakhtiiari, Rezvan Khorshidi, Meisam Omidi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
food.ingredient
Materials science Alginates Cell Survival Composite number 02 engineering and technology 010402 general chemistry 01 natural sciences Gelatin law.invention food Tissue engineering law Shear stress 3D bioprinting Tissue Engineering Tissue Scaffolds Mechanical Engineering Bioprinting Hydrogels General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences On cells Printing Three-Dimensional 0210 nano-technology Biomedical engineering |
| Zdroj: | Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine. 235:314-322 |
| ISSN: | 2041-3033 0954-4119 |
| DOI: | 10.1177/0954411920976682 |
| Popis: | In recent decades, three dimensional (3D) bio-printing technology has found widespread use in tissue engineering applications. The aim of this study is to scrutinize different parameters of the bioprinter – with the help of simulation software – to print a hydrogel so much so that avoid high amounts of shear stress which is detrimental for cell viability and cell proliferation. Rheology analysis was done on several hydrogels composed of different percentages of components: alginate, collagen, and gelatin. The results have led to the combination of percentages collagen:alginate:gelatin (1:4:8)% as the best condition which makes sol-gel transition at room temperature possible. The results have shown the highest diffusion rate and cell viability for the cross-linked sample with 1.5% CaCl2 for the duration of 1 h. Finally, we have succeeded in printing the hydrogel that is mechanically strong with suitable degradation rate and cell viability. |
| Databáze: | OpenAIRE |
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